介质电泳法测定酵母细胞热及特性分析

R. Kido, K. Taguchi
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引用次数: 0

摘要

本文以酵母细胞为靶细胞,用电泳法测定了细胞的热变化。首先,我们进行了酵母细胞在悬浮液中的热损伤评价。这是因为要检查我们提出的实验系统和电极的有效性。结果发现,细胞的活性可以通过并联电压的变化来检测。其次,对酵母细胞热测量进行了验证。通过DEP力将酵母细胞困在电极之间,通过电极间阻抗的变化来测量分路电压的变化。并联电压随温度升高而增大,随温度降低而减小。此外,这种现象只有在细胞被捕获时才得到证实。此外,当捕获的电池数量增加时,电压变化也变大。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cellular Thermal Measurement and Characteristic Analysis of Yeast Cells by Dielectrophoresis
In this paper we measured the cellular thermal changes by dielectrophoresis when we used yeast cell for the target cell. First, we conformed about heat damage evaluation of the yeast cells in the suspension. This is because to check the effectiveness of our proposed experimental systems and our electrodes. As a result, it was found that cells activity could be checked from shunt voltage changes. Second, we conformed about cellular thermal measurement using yeast cell. As the results, yeast cell was trapped between the electrodes by the DEP force and shunt voltage changes were measured as the impedance changes between the electrodes. Shunt voltage increased with the temperature rise, and decreased with the temperature drop. In addition, this phenomenon was confirmed only when the cells have been trapped. Also, when the number of trapped cell increased, voltage changes became larger. 
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