PCR的主设计是一种新型生物标记COVID-19菌株,于2021年1月被确定

M. A. Herli, Dewi Gulyla Hari, Ayu Rahmawati
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摘要

2019冠状病毒病(COVID-19)是一种由严重急性呼吸综合征冠状病毒2 (SARS - CoV-2)引起的传染病。该疾病于2019年12月在中国湖北省武汉市首次传播。SARS-CoV-2具有高度传染性,自发现以来在世界各地迅速蔓延。截至2021年1月,全球共有101,406,059例确诊的SARS-CoV-2感染病例,死亡人数为2191,898人,且仍在增加。COVID-19的诊断有两种常见方法,即快速检测和聚合酶链反应(PCR)。本研究旨在为2021年1月最新分离的COVID-19菌株设计PCR引物,使引物作为COVID-19生物标志物快速准确。结果从2021年1月11日鉴定的新冠病毒(COVID-19)编码为MW454767、MW454771、MW460582、MW454660的最新菌株中获得10条最佳引物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Desain Primer PCR Sebagai Biomarker COVID-19 Strain Terbaru Yang Di Identifikasi Di Januari 2021
Coronavirus disease 2019 (COVID-19) is a contagious disease caused by Severe Acute Respiratory Syndrome Corona Virus 2 (SARS CoV-2). The disease first spread in Wuhan, Hubei Province, China in December 2019. SARS-CoV-2 is highly contagious and has grown rapidly around the world since its discovery. Until January 2021, a total of 101,406,059 confirmed cases of SARS-CoV-2 infection worldwide with a death toll of 2,191,898 and still growing. Diagnosis of COVID-19 is carried out in two common ways, namely a rapid test and PCR (polymerase chain reaction). This study aims to design a PCR primer for the latest COVID-19 strain isolated in January 2021 with the insilico method (computer simulation) so that the primer used as a COVID-19 biomarker is fast and accurate. The results of this study obtained 10 of the best primers from the latest COVID-19 strains with codes MW454767, MW454771, MW460582, MW454660 which were identified on 11 Jan 2021.
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