Pertumbuhan Anggrek Dendrobium Hibrida pada Kultur Cair dengan Penambahan BA dan NAA

Dendrobium is an orchid that has high economic value because of the attractiveness of the color and shape of the flower. High concentrations and limited availability of in vitro culture are needed to obtain large numbers of orchids and their growth in a short time. Liquid MS media with protocorm like bodies (PLB) explants could be used to produce more optimal growth. This study aims to obtain the best combination of BA and NAA on PLB growth in liquid culture and the best PLB yield subcultures on media regeneration. Planting material using PLB age 3 months grown on liquid MS media with a combination treatment of BA and NAA at concentrations of BA (0 mgL -1 , 0.1 mgL -1 , 0.2 mgL -1 ) , NAA (0.5 mgL -1 , 1.0 mgL -1 , 1.5 mgL -1 ). Regeneration media using solid MS with organic ingredients 15% coconut water, 15% potato extract, 15% banana extract 0,2% activated charcoal. The experiment used a completely randomized design with three replications. The results showed that the combination of BA and NAA had a significant effect on the number of PLB lives with the best average being 85.52% and the length of PLB with the best average being 1.27 grams on treatment 0.2 mgL -1 BA + 1.0 mgL -1 NAA.