三和市人宫颈癌随机扩增多态性DNA -RAPD分析

Sumer 1 Pub Date : 2023-08-15 DOI:10.21931/rb/css/s2023.08.01.9

Mohammed Abdulwahab Ati Al-Askeri

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引用次数: 0

摘要

目的:该研究旨在区分与宫颈疾病相关的伊拉克患者基因组DNA中可能存在的遗传变异，并为未来相关应用提供潜在产生的DNA片段作为分子测试的可能性。方法:对宫颈恶性增生和宫颈实变的女性患者进行血液和组织检查;从每个样品中提取和纯化DNA，然后用寡核苷酸“OPA-20”引导“不规则扩增多态性DNA技术”(RAPD)。结果:用寡核苷酸“OPA-20”检测RAPD结果，可以鉴定出女性患者基因组中4个多态性DNA群，而这些DNA群在大多数健康人基因组DNA中缺失。关键词:肿瘤，PCR，伊拉克，基因多态性

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Random amplified polymorphic DNA -RAPD analysis of human cervical cancer in Samwha city

Objectives: The study intended to distinguish a likely hereditary variety in the genomic DNA of Iraqi patients corresponding with cervical disease and the possibility of getting an advantage of the potentially produced DNA fragment(s) as a molecular test for future related applications. Methods: Blood and tissue tests were collected from female patients with cervical malignant growth and solid females; DNA was extricated and purged from each example, and then "Irregular Amplification of Polymorphic DNA technique"(RAPD) was directed with oligonucleotide "OPA-20". Results: RAPD results examination with oligonucleotide “OPA-20” could identify four polymorphic DNA groups in the genome of patients females, in which these bands were missing in the majority of genomic DNA of healthy people. Keywords: Tumor, PCR, Iraq, gene polymorphism

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Sumer 1

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