辐照对代偿恢复性机体反应的剂量依赖性。EPR方法]。

M K Pulatova, D E Filatov, T G Shliakova, V L Sharygin, V P Beletskiĭ, G A Chernov
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引用次数: 0

摘要

研究了生物对大剂量辐射的适应性反应机制。利用EPR光谱分析辐照后犬血、鼠血、脾、骨髓和肝脏代谢变化。结果表明,转铁蛋白和铜蓝蛋白池及核糖核苷酸还原酶活性的动态变化具有相依赖性,在辐照后第2、6和10-12天达到最大值。在不同辐照剂量下观察到这种动力学。这些数据使我们认为生物体的非特异性代偿适应性反应是对辐照的反应。反应强度的剂量-响应函数呈线性关系。剂量-反应曲线的形状表明,生物的最小反应线性依赖于3.2 Gy(狗)以下的剂量和最大剂量。然而,在低剂量辐照(0.25或0.5 Gy)的情况下,最大响应偏离线性,即补偿适应性反应幅度的放大。研究表明,这些效应取决于动物血液的初始个体特征,并与辐照前生物体的“抑制”或“激活”状态有关。用EPR法测定小鼠骨髓和脾脏的核糖核苷酸还原酶活性。从核糖核苷酸还原酶失活的角度讨论了不可修复DNA损伤的性质。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[The dose dependence of the development of compensatory-restorative body reactions to irradiation. The EPR method].

The study deals with the mechanism of organism's adaptive responses to the effect of radiation in widely ranging dose. Post-irradiation metabolic changes were evaluated in canine blood as well as in murine blood, spleen, bone marrow and liver using the EPR spectroscopy. It was shown that the dynamics of changes in transferrin and ceruloplasmin pools and ribonucleotide reductase activity were phase-dependent with the maxima at the 2nd, 6th and 10-12th days after irradiation. Such dynamics was observed at various irradiation doses applied. The data allow us to suggest that the nonspecific compensatory--adaptive reactions of organisms develop as the response to irradiation. The dose-response function of the reaction intensity was found to be linear. The shape of the dose-response curve indicates that the minimum response of organism depends on the dose linearly up to 3.2 Gy (for dogs) as well as the maximum one. However, in the case of low-dose irradiation (0.25 or 0.5 Gy) there were deviations of maximum responses from the linearity, i.e. the amplification of the amplitude of compensatory adaptive reactions. These effect were shown to be dependent upon initial individual characteristics of animal blood and to be related to the "depressed" or "activated" state of organism prior to irradiation. The ribonucleotide reductase activity was measured in bone marrow and spleen of animals by the EPR method. The nature of non-repairable DNA damage is discussed in view of the inactivation of ribonucleotide reductase.

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