Nikmah Ridha Batubara, D. Suryanto, E. Munir, R. Rahmiati
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引用次数: 0
摘要
虾粪是一种易腐烂的物质。降解过程是由腐烂的微生物通过产生降解酶来完成的。虾粪中几丁质含量的存在导致了几丁质酶产菌的产生。几丁质是一种线性均聚物,由n -乙酰- d -葡萄糖胺单体和-(1,4)糖苷键组成。甲壳素具有独特的性质,即;可生物降解,生物相容性好,无毒。这使得几丁质及其衍生物广泛应用于各种工业和生物医学领域,使几丁质及其衍生物具有巨大的经济价值。从虾粪中分离出10株形态特征各异的几丁质溶解菌。分离得到的10株细菌分别为NR02、NR03、NR04、NR05、NR06、NR07、NR08、NR09、NR10、NR11和PU01。4株分离菌产生的几丁质分解指数CI值> 2,即;NR02, NR07, NR09和PU01。NR08产生的IK值最低,为0.01。所获得的几丁质溶解菌具有不同的性状,很可能来自不同的类型
Screening and Characterization of Chitinolytic Bacteria from Shrimp Waste
Shrimp waste is a perishable material. The degradation process is carried out by decaying microbes by producing degrading enzymes. The presence of chitin content in shrimp waste causes the emergence of chitinase enzyme-producing bacteria. Chitin is a linear homopolymer composed of N-acetyl-D-glucosamine monomers with -(1,4) glycosidic bonds. Chitin has unique properties, namely; biodegradable, biocompatible and non-toxic. This causes chitin and its derivatives to be widely used in various industrial and biomedical applications so that chitin and its derivatives have great economic value. There were 10 isolates of chitinolytic bacteria from shrimp waste with varying morphological characteristics. The ten bacterial isolates were NR02, NR03, NR04, NR05, NR06, NR07, NR08, NR09, NR10, NR11 and PU01. Chitinolytic index CI value > 2 was produced by 4 bacterial isolates, namely; NR02, NR07, NR09 and PU01. The lowest IK value was generated by NR08 with a value of 0.01. The chitinolytic bacteria obtained have different characters and it is very likely that they come from different types