ANTIFUNGAL TEST OF THE ETHANOL EXTRACT OF BROTOWALI STEM (Tinospora crispa) ON THE GROWTH OF Trichophyton rubrum IN VITRO

ABSTRACT Background: Trichophyton rubrum is an infectious dermatophyte fungus which is the most common cause of dermatophytosis. Fungal resistance and the side effects of therapy are problems of antifungal agents. Phytochemical test of brotowali stem extract (Tinospora crispa) consist of flavonoids, phenols and triterpenoids which have antifungal effects. This study aimed to examine the effectiveness of brotowali stem extract (Tinospora crispa) as antifungal agent towards the growth of Trichophyton rubrum in vitro using agar well diffusion method. Subjects and Method: This was an experimental study using brotowali stem extract with concentration of 10%, 12,5%, 25%, 30%, 40%, 50%, 60%, 75%, and 100%. The dependent variable was Trichophyton rubrum growth. The independent variable was brotowali stem extract (Tinospora crispa). The data were obtained from the inhibition zone showed in agar well diffusion method in Sabouraud Dextrose Agar media. The data were analyzed using Kruskal-Wallis test. Results: The average diameter of inhibition zone of each variance (10%, 12,5%, 25%, 30%, 40%, 50%, 60%, 75%, and 100%) were 2.167 mm, 6.367 mm, 7.0 mm, 10.67 mm, 119 mm, 13.07 mm, 15.8 mm, 17.96 mm dan 17.13 mm, respectively, and they were statistically significant (p= 0.001). Conclusion: Brotowali stem extract has weak antifungal effectiveness at concentration 10%, 12,5%, 25%, intermediate antifungal effectiveness at concentration 30% and strong antifungal effectiveness at concentration 40%, 50%, 60%, 75% and 100%. Keywords: antifungal, brotowali stem, well diffusion, Trichophyton rubrum Correspondence: Fajriati Zulfa. Faculty of Medicine, Universitas Pembangunan Nasional ‘Veteran’ Jakarta. Jl. RS Fatmawati, Pondok Labu, Jakarta Selatan 12450, Telp. (021) 7656971. Email: nurulnerza@gmail.com DOI: https://doi.org/10.26911/the7thicph.05.02