荧光耦合毛细管电泳技术研究高分子包封量子点及肽段自组装

2016 IEEE International Nanoelectronics Conference (INEC) Pub Date : 2016-05-09 DOI:10.1109/INEC.2016.7589390

Jie Fan, Li Liu, Shumin Ding, M. Fu, Feifei Liu, Chencheng Zhang, Hao Wu, Jianhao Wang, Yuqin Qin

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引用次数: 2

摘要

设计了一种新型生物探针，用于量子点生物分析的研究。然后，利用荧光耦合毛细管电泳(CE-FL)检测双功能聚合物(PAA-g-MEA)包封量子点和ATTO标记肽(ato - ea5h6)的自组装。荧光共振能量转移(FRET)易于检测。FRET信号随着ato - ea5h6 /QD摩尔比的增加而增加，稳定在4:1。

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Studies on polymer capped quantum dots and peptide self-assembly using fluorescence coupled capillary electrophoresis

A new bioprobe was designed to study the quantum dots (QDs) based bioanalysis. Then, fluorescence coupled capillary electrophoresis (CE-FL) was utilized to probe the self-assembly of a bifunctional polymer (PAA-g-MEA) capped QDs and ATTO labeled peptide (ATTO-EA5H6). Fluorescence resonance energy transfer (FRET) was detected readily by CE-FL. The FRET signal increased with ATTO-EA5H6/QD molar ratio, which plateaued at 4:1.

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2016 IEEE International Nanoelectronics Conference (INEC)

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