Molecular detection and risk factors associated with Trichomonas vaginalis infection among pregnant women in Osogbo and Ede, Southwest Nigeria

Background: Trichomoniasis is the most prevalent curable sexually transmitted disease globally. This study provides information on the prevalence and risk factors of Trichomoniasis among pregnant women in primary healthcare centres in Osogbo and Ede areas in Osun State, Nigeria. Methodology: High vaginal swab samples were obtained from 145 consenting pregnant women using sterile swab sticks and analysed through microscopic examination and PCR diagnostic techniques. Questionnaires were administered to investigate the participants’ socio-demographic characteristics, hygiene practices, and knowledge and practices (KAP) about trichomoniasis. Result: The overall prevalence of trichomoniasis was 0%, as no trophozoite was detected in any sample. There was no statistical difference between the number of sexual partners, occupation, family type, and previous sexually transmitted infections. Many of the respondents (89.7%) were ignorant of this disease and its transmission mode. Conclusion: The molecular technique employed in this study has further confirmed the zero prevalence of trichomoniasis in the study area and may be a promising diagnostic test.