I. Ullah, B. Shahid, Muhammad Ijaz Khan, S. Jalali, K. Farooq, Akbar Ali
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引用次数: 0
摘要
本研究旨在探讨l -抗坏血酸(AsA)和2巯基乙醇(2-ME)对水牛积云封闭卵母细胞(ceo)和积云剥脱卵母细胞(cdo)体外成熟的抗氧化作用。对照组采用不添加任何培养基。在处理组中，培养基以以下组合补充(i) 100M H2O2 (ii) 100M H2O2 + 25 M 2-ME + 250 M AsA (iii) 100M H2O2 + 25 M 2-ME (iv) 100M H2O2 + 250 M AsA。在没有H2O2的情况下，24小时的退化程度较低。复合添加剂减少了卵母细胞的变性，并将MII cdo的比例提高到与CEO相似的水平。卵丘细胞保护卵母细胞免受氧化应激。在培养基中添加100M H2O2 + 25 M 2-ME + 250 M AsA，培养脱毛卵母细胞，可有效对抗氧化应激。
Combination of L-Ascorbic Acid and 2- Mercaptoethanol Reduces the Damaging Effect of Hydrogen Peroxide During in Vitro Maturation of Nili Ravi Buffalo Denuded Oocytes
The objective of this study was to evaluate the anti-oxidative effect of L-ascorbic acid (AsA) and 2mercaptoethanol (2-ME) on in-vitro maturation of Nili Ravi buffalo cumulus enclosed oocytes (CEOs) and cumulus denuded oocytes (CDOs). The medium without any supplementation was used in control group. In treatment groups the medium was supplemented in following combinations (i) 100 M H2O2 (ii) 100M H2O2 + 25 M 2-ME + 250 M AsA (iii) 100M H2O2 + 25 M 2-ME (iv) 100M H2O2 + 250 M AsA. In absence of H2O2 the degeneration at 24 hrs was lower. The combined additives reduced the degeneration of oocytes and increased the proportion of MII CDOs to similar levels to those achieved by CEO. Cumulus cells protect oocytes from oxidative stress. The supplementation of medium with 100M H2O2 + 25 M 2-ME + 250 M AsA, for the culture of denuded oocytes effectively counteracts the oxidative stress.
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