{"title":"苯酚-硫酸法测定大鼠骨骼肌和肝脏中糖原含量的研究","authors":"A.M. Tretyakova, N. Vakhnina","doi":"10.34014/mpphe.2021-189-191","DOIUrl":null,"url":null,"abstract":"The possibility of using the phenol-sulfuric acid method for the determination of total glycogen, its acid-soluble and acid-insoluble fractions in the liver and skeletal muscles of rats was studied. It was found that the use of a precipitant in the isolation of total glycogen and its fractions increases the yield of the investigated substances. \nKey words: phenol-sulfate method, rats, liver, muscles, total glycogen, acid-soluble glycogen, acid-insoluble glycogen.","PeriodicalId":378573,"journal":{"name":"Materialy VIII Vserossijskoj konferencii s mezhdunarodnym uchastiem «Mediko-fiziologicheskie problemy jekologii cheloveka»","volume":"6 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of the phenol-sulfuric acid method for the determination of glycogen in skeletal muscles and liver of rats\",\"authors\":\"A.M. Tretyakova, N. Vakhnina\",\"doi\":\"10.34014/mpphe.2021-189-191\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The possibility of using the phenol-sulfuric acid method for the determination of total glycogen, its acid-soluble and acid-insoluble fractions in the liver and skeletal muscles of rats was studied. It was found that the use of a precipitant in the isolation of total glycogen and its fractions increases the yield of the investigated substances. \\nKey words: phenol-sulfate method, rats, liver, muscles, total glycogen, acid-soluble glycogen, acid-insoluble glycogen.\",\"PeriodicalId\":378573,\"journal\":{\"name\":\"Materialy VIII Vserossijskoj konferencii s mezhdunarodnym uchastiem «Mediko-fiziologicheskie problemy jekologii cheloveka»\",\"volume\":\"6 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Materialy VIII Vserossijskoj konferencii s mezhdunarodnym uchastiem «Mediko-fiziologicheskie problemy jekologii cheloveka»\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.34014/mpphe.2021-189-191\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materialy VIII Vserossijskoj konferencii s mezhdunarodnym uchastiem «Mediko-fiziologicheskie problemy jekologii cheloveka»","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.34014/mpphe.2021-189-191","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Application of the phenol-sulfuric acid method for the determination of glycogen in skeletal muscles and liver of rats
The possibility of using the phenol-sulfuric acid method for the determination of total glycogen, its acid-soluble and acid-insoluble fractions in the liver and skeletal muscles of rats was studied. It was found that the use of a precipitant in the isolation of total glycogen and its fractions increases the yield of the investigated substances.
Key words: phenol-sulfate method, rats, liver, muscles, total glycogen, acid-soluble glycogen, acid-insoluble glycogen.
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