不同盐度和氮浓度下亚酱螺旋藻的生长和色素

Lolymar Romero Maza, M. Guevara, J. Bernal
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引用次数: 1

摘要

研究了一株新分离的螺旋藻(Spirulina subsalsa)在不同盐度和氮浓度海水中的生长和色素含量。从委内瑞拉苏克雷州克拉韦利诺水库的水样中分离出新的S. subsalsa菌株,并使用Aguiar(2013)提出的分类密钥进行鉴定。所测试的培养基为f / 2吉拉德,修改硝酸盐浓度(14.5;29、59 mmol/L)和氯化钠(加入海水0,9和18‰)。在受控环境(温度:30±1°C,照度:3000勒克斯,手动搅拌,光周期12:12)下,不连续地进行三次培养,持续21天。结果表明:盐度为9‰、硝酸盐浓度为14 mmol/L时生物量最高;相同氮浓度下,叶绿素a和藻蓝蛋白在0‰时较高。这些结果表明,盐度和氮浓度影响了新分离菌株的生长和色素,表明该菌株具有生物技术开发潜力,可以为食品和药物工业获得有价值的代谢物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Crecimiento y pigmentos de Spirulina subsalsa cultivada a diferentes salinidades y concentraciones de nitrógeno
The aim of this research was to evaluate the growth and content of pigments of a new isolate of Spirulina subsalsa grown in seawater at different salinities and nitrogen concentrations. The new S. subsalsa strain was isolated from water samples from the Clavellino reservoir, Sucre state, Venezuela, and was identified using the taxonomic key proposed by Aguiar (2013). The culture medium tested was f / 2 Guillard, modifying the concentrations of nitrate (14.5; 29, 59 mmol/L) and sodium chloride (0, 9 and 18 ‰ by the addition of seawater). The cultures were carried out in triplicate, discontinuously, under conditions of controlled environment (T: 30±1 °C, illumination: 3,000 lux, manual agitation, photoperiod 12:12), for 21 days. The results obtained showed that the salinity of 9 ‰ and the nitrate concentration of 14 mmol/L were the parameters that propitiated the highest biomass; while chlorophyll a and phycocyanin showed higher values ​​at the same nitrogen concentration at 0 ‰. These findings indicate that salinity and nitrogen concentration affect the growth and pigments of the new isolate of S. subsalsa and suggest that this strain has potential for its biotechnological exploitation in order to obtain valuable metabolites for food and pharmacological industries.
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