银杏叶提取物对成年雄性白化大鼠甲状腺的影响:组织学、组织化学和形态计量学研究

R. Bushra
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引用次数: 0

摘要

简介:银杏叶提取物(EGb)已使用多年作为膳食补充剂和草药治疗范围广泛的疾病。然而,人们发现它与副作用有关。应该进行研究,以提供有关其在医学上安全性的信息。研究目的:探讨两种不同剂量EGb对成年雄性白化大鼠甲状腺功能、组织学、组织化学和形态计量学水平的影响。材料与方法:体重200 ~ 250 g的成年雄性白化大鼠30只,随机分为3组。第1组(对照组,n=10)给予蒸馏水。低剂量组(n=10)给予100 mg EGb /kg b.w。第三组(高剂量组,n=10)给予200 mg EGb /kg b.w。治疗方法均为口服,每日灌胃,连续4周。在研究的指定时间,取血检测血清(T3, T4和TSH)。麻醉后,取大鼠瘢痕,解剖甲状腺叶,进行光镜和电镜检查。记录各组卵泡上皮高度、卵泡直径和胶体面积%平均值并进行比较。结果:与对照组相比,EGb处理的动物(2组和3组)血清T3和T4水平显著降低,TSH水平显著升高。与对照组相比,2组和3组卵泡细胞高度显著增加,卵泡直径和胶体面积%显著降低。卵泡细胞不规则、卵泡细胞肥大和增生、卵泡核深度染色、内质网和高尔基体扩张、线粒体和溶酶体改变、细胞质空泡化和间质毛细血管扩张。这些变化在第三组更为明显。正常分泌活性(对照组)的滤泡细胞出现了一周的基底膜PAS反应和中度的胶体PAS反应,而2组和3组的无活性滤泡细胞在基底膜出现了中度PAS反应和明显的胶体PAS反应。我们的结果表明,EGb管理导致甲状腺功能减退的状态。结论:在使用EGb作为必要治疗的情况下应注意。仅供个人非商业用途。解剖学版权©2018。版权所有DOI: 10.21608/EJANA.2021.171456收稿日期:2017年6月1日,接收日期:2016年6月16日
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Ginkgo Biloba Extract Administration on the Thyroid Gland of the Adult Male Albino Rat: A Histological, Histochemical and Morphometric Study
Introduction: Ginkgo biloba extract (EGb) has been used for many years as a dietary supplement and as a herbal treatment for a wide range of diseases. However, it was found to be associated with adverse effects. Studies should be conducted to provide an information on its safety in medicine. Aim of the Work: The goal of this study was to investigate the effects of two different doses of EGb on the thyroid gland of the adult male albino rat on the functional, histological, histochemical and morphometric levels. Material and Methods: Thirty adult male albino rats each weighing (200-250 g) were categorized into three equal groups. Group 1(control, n=10) were given distilled water. Group 2 (low dose, n=10) was given (100 mg EGb /kg b.w.). Group 3 (high dose, n=10) was given (200 mg EGb /kg b.w.). All the treatment was given orally by daily gavage for 4 weeks. At the designated time of the study, blood samples were taken for determination of serum (T3, T4 and TSH). After anesthesia, rats were scarified and thyroid lobes were dissected and processed for light and electron microscopic examinations. Follicular epithelial height, follicular diameter and colloid area % means of all groups were registered and compared. Results: EGb treated animals (groups 2 and 3) demonstrated a significant decreased serum levels of T3 and T4 and a significant increase of TSH levels when compared to the control group. The follicular cells height was significantly increased, while the follicular diameter and colloid area % were significantly decreased in groups (2&3) on comparing to control. The EGb-treated animals showed histological changes in the form of irregular follicular cells, follicular cell hypertrophy and hyperplasia, deeply stained follicular nuclei, dilated profiles of rER and Golgi apparatus, mitochondrial and lysosomal alterations, vacuolated cytoplasm and dilated interstitial blood capillaries. These changes were more pronounced in the group 3. While the follicular cells of the normally secretory active (control group) revealed a week basal lamina PAS reaction and a moderate colloid PAS reaction, the inactive follicles of groups 2 and 3 showed a moderate PAS reaction in the basal follicular lamina and a marked colloid PAS reaction. Our results demonstrated that EGb administration leads to a state of hypothyroidism. Conclusion: Considerations should be taken in cases where the EGb is used as a necessary treatment. Personal non-commercial use only. Anatomy copyright © 2018. All rights reserved DOI: 10.21608/EJANA.2021.171456 Received: 01 June 2017, Accepted: 16 June 2016
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