糖尿病口腔念珠菌生物膜形成、毒力因子及抗真菌敏感性的研究

L. Yamauchi
{"title":"糖尿病口腔念珠菌生物膜形成、毒力因子及抗真菌敏感性的研究","authors":"L. Yamauchi","doi":"10.46889/jcim.2022.3306","DOIUrl":null,"url":null,"abstract":"Background: Candida species colonize human microbiota and some conditions, such as immunosuppression or chronic illness, predispose the individual to fungal infections; among them, diabetes mellitus, a metabolic disorder frequently associated with higher rates of yeast infections.\n\nMaterial and Methods: The prevalence of Candida species in the oral cavity of patients with diabetes mellitus was evaluated and the carriage was compared between type 1 and type 2 diabetic groups. In addition, in vitro susceptibility to antifungals, biofilm formation, cell surface hydrophobicity, and the production of hydrolytic enzymes were tested.\n\nResults: The results demonstrated the presence of different Candida species in the oral cavity of diabetic patients; and, also showed that type 1 diabetic patients are more susceptible to Candida colonization. Almost all isolates produce virulence factors such as proteases, phospholipases, or form biofilm; and they are sensitive to fluconazole and nystatin.\n\nConclusion: Colonization of Candida spp. oral isolates from type 1 and type 2 diabetic patients were similar; however, type 1 presented a higher colony-forming unit counting. Overall, Candida isolates from the oral cavity of diabetic patients are potential pathogens of candidiasis.","PeriodicalId":308430,"journal":{"name":"Journal of Clinical Immunology & Microbiology","volume":"87 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biofilm Formation, Virulence Factors and Antifungal Susceptibility of Candida spp. Isolated From the Oral Cavity of Diabetes Mellitus Patients\",\"authors\":\"L. Yamauchi\",\"doi\":\"10.46889/jcim.2022.3306\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Candida species colonize human microbiota and some conditions, such as immunosuppression or chronic illness, predispose the individual to fungal infections; among them, diabetes mellitus, a metabolic disorder frequently associated with higher rates of yeast infections.\\n\\nMaterial and Methods: The prevalence of Candida species in the oral cavity of patients with diabetes mellitus was evaluated and the carriage was compared between type 1 and type 2 diabetic groups. In addition, in vitro susceptibility to antifungals, biofilm formation, cell surface hydrophobicity, and the production of hydrolytic enzymes were tested.\\n\\nResults: The results demonstrated the presence of different Candida species in the oral cavity of diabetic patients; and, also showed that type 1 diabetic patients are more susceptible to Candida colonization. Almost all isolates produce virulence factors such as proteases, phospholipases, or form biofilm; and they are sensitive to fluconazole and nystatin.\\n\\nConclusion: Colonization of Candida spp. oral isolates from type 1 and type 2 diabetic patients were similar; however, type 1 presented a higher colony-forming unit counting. Overall, Candida isolates from the oral cavity of diabetic patients are potential pathogens of candidiasis.\",\"PeriodicalId\":308430,\"journal\":{\"name\":\"Journal of Clinical Immunology & Microbiology\",\"volume\":\"87 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Clinical Immunology & Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.46889/jcim.2022.3306\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Immunology & Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.46889/jcim.2022.3306","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景:念珠菌在人体微生物群中定植,某些情况下,如免疫抑制或慢性疾病,使个体易患真菌感染;其中,糖尿病是一种代谢紊乱,通常与较高的酵母菌感染率有关。材料与方法:对1型和2型糖尿病患者口腔内念珠菌的流行情况进行评估,并比较2型糖尿病患者口腔内念珠菌的携带情况。此外,还测试了抗真菌药物的体外敏感性、生物膜的形成、细胞表面疏水性和水解酶的产生。结果:糖尿病患者口腔中存在不同种类的念珠菌;研究还表明,1型糖尿病患者更容易被念珠菌定植。几乎所有的分离株都产生毒力因子,如蛋白酶、磷脂酶或形成生物膜;对氟康唑和制霉菌素敏感。结论:1型和2型糖尿病患者口腔念珠菌的定殖相似;而1型菌落形成单位计数较高。总之,从糖尿病患者口腔分离的念珠菌是潜在的念珠菌病病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biofilm Formation, Virulence Factors and Antifungal Susceptibility of Candida spp. Isolated From the Oral Cavity of Diabetes Mellitus Patients
Background: Candida species colonize human microbiota and some conditions, such as immunosuppression or chronic illness, predispose the individual to fungal infections; among them, diabetes mellitus, a metabolic disorder frequently associated with higher rates of yeast infections. Material and Methods: The prevalence of Candida species in the oral cavity of patients with diabetes mellitus was evaluated and the carriage was compared between type 1 and type 2 diabetic groups. In addition, in vitro susceptibility to antifungals, biofilm formation, cell surface hydrophobicity, and the production of hydrolytic enzymes were tested. Results: The results demonstrated the presence of different Candida species in the oral cavity of diabetic patients; and, also showed that type 1 diabetic patients are more susceptible to Candida colonization. Almost all isolates produce virulence factors such as proteases, phospholipases, or form biofilm; and they are sensitive to fluconazole and nystatin. Conclusion: Colonization of Candida spp. oral isolates from type 1 and type 2 diabetic patients were similar; however, type 1 presented a higher colony-forming unit counting. Overall, Candida isolates from the oral cavity of diabetic patients are potential pathogens of candidiasis.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信