基于原子力显微镜的猪OBP膜人工嗅觉研究

2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society Pub Date : 2001-10-25 DOI:10.1109/IEMBS.2001.1019569

Q. Cai, M. Pan, Guang Li, Yuquan Chen

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引用次数: 0

摘要

嗅觉结合蛋白(OBP)在嗅觉信号传递中起着重要作用。本文研究了OBP在仿生人工鼻子上的潜在应用。最重要的是，OBP是固相固定的。因此制备了两种OBP薄膜。首先，利用dtc作为交联剂将OBP共价固定在硅片上。其次，采用电化学聚合的方法在ITO玻璃表面制备聚吡咯薄膜。利用原子力显微图(AFM)测量了这些薄膜的形貌。为了评估OBP及其抗体的亲和力，采用共价法将抗OBP包被在AFM探针上。分析了原子力显微镜获得的力距曲线，揭示了薄膜的特性以及OBP与抗体的结合特性。

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An AFM-based study of porcine OBP film for artifical olfaction

The odorant binding protein (OBP) plays an important role in olfactory signal transmission. This paper studies the potential application of OBP on bionic artificial nose. Above all, OBP is immobilized in solid phase. So two kinds of OBP film were prepared. Firstly, OBP is covalently immobilized on the silicon wafer using DITC as a crosslinker. Secondly, polypyrrole film was fabricated on the ITO glass by the electrochemical polymerization. The topograph of these films was measured using an Atomic Force Micrograph (AFM). In order to evaluate the affinity of OBP and its antibody, the AFM probe was coated with the anti-OBP using the covalent method. The force-distance curve, acquired by the AFM instrument, was analyzed to reveal the characteristics of thin film and the binding property between OBP and antibody.

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2001 Conference Proceedings of the 23rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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