TO EVALUATE THE PROTECTIVE EFFECT OF PROTEIN OF PIPER LONGUM LINN ON CELL VIABILITY OF HUMAN PERIPHERAL LYMPHOCYTES AGAINST DAMAGE INDUCED BY HYDROGEN PEROXIDE AND TERTIARY BUTYL HYDROPEROXIDE

A major alkaloid, piperine is present in P.longum which showed significant anti-metastasis activity. Chemopreventive effects are also shown by piperine when it is administrated orally on the animals suffering from lung cancer. The fruits were air dried pulverized and used for analysis. Preliminary screening of secondary metabolites. Natural plants like long pepper are the main sources of bioactive molecules and have played a major role in discovery of lead compounds .The curative properties of medicinal plants of long pepper are due to the presence of various secondary metabolites such as alkaloids, flavonoids, glycosides, phenols, saponins, sterols etc. The bio enhancing effect of pepper species in traditional as well as modern medicine may be due to two major mechanisms; first, because of a nonspecific mechanism in which the rapid absorption of the drug may be observed by the decreased secretion of HCl. Despite the outstanding potential of piperine reported in pre-clinical studies, no clinical trials are ongoing in cancer patients. The bio enhancing properties of piperine are being explored in a clinical trial in association with curcumin to assess whether the combination may reduce inflammation and discomfort from a ureteric stent in cancer patients