检测Wi-Fi波对蚕豆表型和分子标记的影响。

Rawad Khalaf Hameed, R. Z. Al-sugmiany, Hisham Majeed Shlash, Maan Hasan Salih
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摘要

该研究的目的是评估蚕豆暴露于Wi-Fi波对其生长和分子水平的影响。将蚕豆种子浸泡48 h,将种子分为5组(处理4组,对照组1组),记录种子的表型特征,同时利用RAPD-PCR分子标记检测波浪对DNA序列的影响。形态研究中,B处理株高最高,为123.9,其次是C、D、E。豆荚数最高,为13.6,其次是D、C、B。种子数最高,为34.4,其次是D、B、C处理。叶片数以B处理最多,为251片,其次为C、D、E处理。叶片测量结果显示,E处理叶面积最大,为3.2 ~ 6.1,D、C、B处理次之。大部分处理的突变条带均以独特条带和缺失条带(51条)、独特条带(33条)和缺失条带(18条)区分,处理E (60) D获得的独特条带数量较多,为(12条),处理B (15) D获得的条带数量较多,为(4条),而缺失条带处理D (45) D获得的缺失条带数量较多,为(6条),处理B (15) D获得的缺失条带数量较多,为(4条)。形态学标记和分子标记的结果存在差异,这是由于它们的编码DNA和非编码DNA的基因组突变频率不同。Wi-Fi波诱导蚕豆(Vicia faba L.)基因组发生突变,且突变随Wi-Fi波暴露时间的延长而增加。RAPD标记对蚕豆基因组产生突变的检测效率较高
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detecting the effects of Wi-Fi waves on phenotypic and molecular markers of Vicia faba L.
The study objective was to evaluate the effects of exposure Vicia faba to Wi-Fi waves on growth and molecular levels. The seeds of the Vicia faba plant were soaked for 48 hours, then the seeds were divided into five groups, four for treatments and one for the control group, phenotypic characteristics were recorded, while the molecular markers were evaluated using RAPD-PCR markers to detect the effect of waves on DNA sequence. Morphological study, the highest plant height recorded in treatment B that was 123.9 followed by C, D and E. As for the bean pod number, the treatment E was 13.6 that showed the highest number that followed by D, C and B. For the seed number, the highest was E 34.4 seed that followed by D, B and C treatments. The number of leaves in the plants, the highest was in treatment B that was 251 followed by C, D and E treatments respectively. The leaf measurements recorded, the highest leaf area was in the treatment E that was 3.2-6.1 followed by D, C and B treatments respectively. Most of the treatments were distinguished by unique bands and absent of all mutant bands (51), unique bands (33) and absent bands (18), the treatment E (60) day got a higher number of unique bands that were (12), and the treatment B (15) day have got (4) bands, while the absent bands the treatment D (45) day got a higher number of the absent bands that were (6), and the treatment B (15) day got (4) bands. There was a difference in the results between the morphological and molecular markers because of the difference in mutation frequency on the genome of their coding and noncoding DNA. The Wi-Fi waves induced mutations in the genome of Vicia faba L. and the mutations increased with the period of exposure of the plant to the Wi-Fi waves. The efficiency of the RAPD markers was high in detecting the producing mutation in the genome of Vicia faba L
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