Pedro Eduardo Moreno-Zacarías, Flor Del Carmen Ramos-Dueñas
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引用次数: 0
摘要
使用天然的氮唑(Chenopodium ambrosioides L.)提取物,已被证明可以控制植物性真菌(P. Aguilar et al., 2013;(J. Black Solis等,2017)。采用完全随机试验设计,将1 ml浓度为25%、50%、100%的乙醇提取物用无菌蒸馏水(V:V)稀释后,分别加入PDA培养皿中抑制尖孢镰刀菌和茄枯菌的胶束发育,采用方差分析和均数与Tukey’s倍数范围的比较检验进行统计学分析。经过8天的培养,两种真菌的抑菌效果均达到100%,菌落平均直径为62 mm。而对尖孢镰刀菌和茄枯镰刀菌分别为62 mm和61 mm,差异有统计学意义(p≤0.05)。胶束抑制发育的浓度分别为25%和50%,但两种真菌的胶束抑制浓度之间没有统计学差异(p≥0.05)。结果表明,epazote乙醇提取物可用于防治这两种植物病原真菌。
Evaluation of inhibitor effect on micelium development on Fusarium oxysporum, y F. solani, using three dosis of epazote epazote (Chenopodium ambrosioides L.)
Using natural extract of epazote (Chenopodium ambrosioides L.), has shown to control phytopatogenic fungi (P. Aguilar et al., 2013; y J. Black Solis et al., 2017). One mililiter of ethanolic extract at concentrations of 25%, 50%, 100% diluted on sterile distilled water (V:V) has been used to inhibit micelial developement of Fusarium oxysporum, and Fusarium solani, added to PDA Petri dish under a completely randomized experimental design, a statistical analysis was carried out by means of an ANOVA and comparison test of means with Tukey´s multiple range. After eight days of incubation, the outstanding inhibit effect has been observed with 100% and the average diameter of colonies was 62 mm., has been measured on both fungi species. In contrast, 62 mm., and 61 mm., was observed on F. oxysporum and F. solani respectively, and a statistical effect was observed (p≤0.05). With 25% and 50%, micelial inhibit developement has been measured, but no statistical differences between concentrations has been calculated (p≥0.05) for both fungi. The results suggest that ethanolic extract of epazote could be used to control both phytopathogenic fungi.