{"title":"两种免疫诊断技术检测牛支原体亚种的比较","authors":"M. Aliyu, G. Egwu, J. Ameh","doi":"10.4314/TV.V21I1.4524","DOIUrl":null,"url":null,"abstract":"The use of Dot-Enzyme immunoassay (Dot-EIA) and Avidin-Biotin \n\nComplex (ABC-Elite) test in detecting Mycoplasma mycoides subspecies mycoides Small colony (MmmSc) in CBPP suspect tissues was evaluated. Out of the 330 suspect lung tissues and 20 suspect lymph nodes tissue samples analyzed, 245 (75.9%) and 276 (83.3%) of the lung tissues were positive by ABC-Elite and Dot-EIA respectively. However, the result of 11(3.45%) and 10(3.0%) of the lung tissues samples analyzed by ABC Elite and Dot EIA respectively could not be interpreted hence were considered to be doubtful. It is concluded that while both Dot-EIA and BC-Elite could be used efficiently in the field Dot-EIA is considered superior because of its cost-effectiveness, accuracy and speediness of results compared to ABC-Elite method. \n(Tropical Veterinarian: 2003 21(1): 23-29)","PeriodicalId":428776,"journal":{"name":"Tropical veterinarian","volume":"60 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Comparison of two immune diagnostic techniques for detection of Mycoplasma mycoides sub species mycoides SC in cattle\",\"authors\":\"M. Aliyu, G. Egwu, J. Ameh\",\"doi\":\"10.4314/TV.V21I1.4524\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The use of Dot-Enzyme immunoassay (Dot-EIA) and Avidin-Biotin \\n\\nComplex (ABC-Elite) test in detecting Mycoplasma mycoides subspecies mycoides Small colony (MmmSc) in CBPP suspect tissues was evaluated. Out of the 330 suspect lung tissues and 20 suspect lymph nodes tissue samples analyzed, 245 (75.9%) and 276 (83.3%) of the lung tissues were positive by ABC-Elite and Dot-EIA respectively. However, the result of 11(3.45%) and 10(3.0%) of the lung tissues samples analyzed by ABC Elite and Dot EIA respectively could not be interpreted hence were considered to be doubtful. It is concluded that while both Dot-EIA and BC-Elite could be used efficiently in the field Dot-EIA is considered superior because of its cost-effectiveness, accuracy and speediness of results compared to ABC-Elite method. \\n(Tropical Veterinarian: 2003 21(1): 23-29)\",\"PeriodicalId\":428776,\"journal\":{\"name\":\"Tropical veterinarian\",\"volume\":\"60 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1900-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tropical veterinarian\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/TV.V21I1.4524\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tropical veterinarian","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/TV.V21I1.4524","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
评价斑点酶免疫分析法(Dot-EIA)和亲和素-生物素复合物(ABC-Elite)检测疑似CBPP组织中支原体亚种mycoides Small colony (MmmSc)的作用。在330例疑似肺组织和20例疑似淋巴结组织中，ABC-Elite阳性245例(75.9%)，Dot-EIA阳性276例(83.3%)。然而，ABC Elite和Dot EIA分析的肺组织样本中分别有11例(3.45%)和10例(3.0%)的结果无法解释，因此被认为是可疑的。结果表明，Dot-EIA和BC-Elite方法均可在野外有效应用，但与ABC-Elite方法相比，Dot-EIA方法具有成本效益、结果准确性和快速性。(热带兽医:2003 21(1):23-29)
Comparison of two immune diagnostic techniques for detection of Mycoplasma mycoides sub species mycoides SC in cattle
The use of Dot-Enzyme immunoassay (Dot-EIA) and Avidin-Biotin
Complex (ABC-Elite) test in detecting Mycoplasma mycoides subspecies mycoides Small colony (MmmSc) in CBPP suspect tissues was evaluated. Out of the 330 suspect lung tissues and 20 suspect lymph nodes tissue samples analyzed, 245 (75.9%) and 276 (83.3%) of the lung tissues were positive by ABC-Elite and Dot-EIA respectively. However, the result of 11(3.45%) and 10(3.0%) of the lung tissues samples analyzed by ABC Elite and Dot EIA respectively could not be interpreted hence were considered to be doubtful. It is concluded that while both Dot-EIA and BC-Elite could be used efficiently in the field Dot-EIA is considered superior because of its cost-effectiveness, accuracy and speediness of results compared to ABC-Elite method.
(Tropical Veterinarian: 2003 21(1): 23-29)
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