番茄叶面细菌抗真菌活性的研究

B. Saleem, P. K. Paul
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引用次数: 1

摘要

各种各样的细菌和微真菌物种都具有一些特征,比如分泌代谢化合物或毒素,帮助它们相互作用或抑制它们的生长。本研究从番茄叶面中分离细菌,对其对同一生境中分离的微真菌进行抑菌性能测试。采用叶片印迹技术分离细菌和真菌,分别在35±1℃和25±1℃条件下在营养琼脂和马铃薯葡萄糖琼脂上进行纯培养。采用双培养法测定其拮抗活性。结果表明,高丽假单胞菌、斯图茨氏假单胞菌、大全氏鞘菌和肺炎克雷伯菌抑制了番茄根丝胞菌、互交孢菌、柑橘芽胞菌、扩张青霉、尖孢镰刀菌、草本枝孢菌、枝孢霉、月曲霉、黄曲霉和假单胞菌的生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antifungal Activity of Bacteria on Phylloplane of Tomato
A wide array of bacterial and microfungal species possess features, like secretion of metabolic compounds or toxins that help them in interaction with each other or suppression of their growth. In the present study, bacteria were isolated from the phylloplane of Lycopersicum esculentum, to evaluate their antifungal properties tested against microfungal species isolated from the same habitat. Bacterial and fungal isolates were obtained by the leaf imprint technique and pure cultures were made on nutrient agar and potato dextrose agar at 35±1°C and 25±1°C, respectively. The antagonistic activity was tested by dual culture assay. It was found that the growth of Rhizoctonia solani, Alternaria alternata, A. citrifolia, Penicillium expansum, Fusarium oxysporum, Cladosporium herbarum, C. cladosporioides, Curvularia lunata, Aspergillus flavus and A. candidus was inhibited by Pseudomonas koreensis, P. stutzeri, Sphingobacterium daejeonense and Klebsiella pneumonea.
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