用杆菌小脑菌测试森林抗菌活性

Supartiningsih Supartiningsih, Jon Kennedy Marpaung, Yosy Silalahi, Icha Wahyuni Turnip
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引用次数: 1

摘要

蜂蜜是一种粘稠的甜液体,来自蜜蜂,可以治疗许多疾病,并具有抗菌作用。蜂蜜的抑菌潜力由活性成分含量、低水分、高渗透压、双氧水含量和低ph决定。本研究旨在测定森林蜂蜜在多洛克萨里布、斯马伦贡地区对蜡样芽孢杆菌生长的抑菌活性。实验研究方法包括分析蜂蜜的质量,包括检查水分含量、灰分含量、pH值、酸度、粘度、比重、植物化学筛选,并采用圆盘扩散法进行抗菌活性试验,采用空白圆盘,每种浓度分别为25%、35%、45%、55%和65%,阳性对照采用红霉素,阴性对照采用无菌蒸馏水。对森林蜂蜜进行了感官测试,结果表明蜂蜜达到了良好的质量要求。对蜂蜜进行SNI 3545: 2013的水分灰分测试和pH值测试结果。浓度为25%、35%、45%、55%和65%的森林蜂蜜对蜡样芽孢杆菌的抑菌活性试验表明,在10、16 mm、13 mm、15、6 mm、21、6 mm和22、6 mm的空白圆盘周围均有抑菌作用。对森林蜂蜜的抑制作用在浓度最高时达到65% (22.6 mm)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UJI AKTIVITAS ANTIBAKTERI MADU HUTAN TERHADAP BAKTERI Bacillus cereus
Honey is a thick sweet liquid which comes from bees which can cure many diseases and is antibacterial. Antibacterial potential of honey caused by the content of active compounds, low moisture content, high osmolarity, hydrogen peroxide content, and low pH.The aim of this study was to determine the antibacterial activity of forest honey on Doloksaribu, Simalungun district on the growth of Bacillus cereus bacteria.Experiment research method includes the analysis quality of honey, includes checking moisture content, ash content, pH tested, acidity, viscosity, spesific gravityphytochemical screening, and antibacterial activity test ith disk diffusion method use blank disk with each concentration 25%, 35%, 45%, 55%, and 65%, positive control using erythromycin and negative control sterile distilled water. Organoleptic test conducted on forest honey gave results that honey reach the requirements for good quality. The result of moisture and ash content test and pH test reach of SNI 3545: 2013 about honey. Antibacterial activity test of forest honey with concentration 25%, 35%, 45%, 55%, and 65% against Bacillus cereus showed that there was an inhibitory around the blank disc, namely 10,16 mm, 13 mm, 15,6 mm, 21,6 mm, and 22,6 mm. The greatest inhibition of forest honey occurred at the highest concentration that is 65% (22,6 mm).
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