用时间分辨步进扫描FTIR光谱监测蛋白质反应

K. Gerwert
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引用次数: 0

摘要

时间分辨步进扫描FTIR差分光谱允许以纳秒时间分辨率在原子分辨率下监测蛋白质反应。该技术监测假基的反应,如顺反异构化,蛋白质主链的运动和氨基酸的质子化变化或氢键的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein Reactions Monitored by Time-Resolved Step-Scan FTIR Spectroscopy
Time-resolved step-scan FTIR difference-specroscopy allows monitoring of protein-reactions with nanoseconds time-resolution at atomic resolution. The technique monitors reaction of prosthetic groups, like cis-trans isomerization, movements of the protein backbone and protonation changes or H-bond changes of aminoacids.
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