Endemic Bacteria Potential for Decomposing Pesticide Prophenophos and Chlorantraniliprole from Pesticide-Exposed Soybean Field in East Java

Pest control in soybean field in general still uses synthetic pesticide. Soil bacteria from pesticide-exposed field are potentially able to decompose pesticide residue in during certain period of time. This research aimed to find out the potency of isolated endemic bacteria from soybean field in Jombang, Lamongan, and Probolinggo in East Java for decomposing pesticide prophenophos and chlorantraniliprole. This research used observation method. Bacteria sample was obtained from soybeans fields exposed by pesticide in nutrient agar medium with addition prophenophos and chlorantraniliprole using pour plate method. Testing medium used was nutrient agar with added prophenophos (1 g/l) and chlorantraniliprole (2 g/l), in which bacteria was incubated for 1 day. The area of the clear zone was determined by subtraction of clear zone diameter with well diffusion diameter. Results showed that endemic soil bacteria isolates grown in medium with addition of prophenophos showed clear zone were amounted to15 (Jombang), 19 (Lamongan), and 16 (Probolinggo), while in chlorantraniliprole-added medium were 21 (Jombang), 23 (Probolinggo), and 25 (Lamongan) isolates. The total number of endemic bacteria isolates obtained from the soybean fields in those 3 areas able to decompose prophenophos was 50 isolates, while clorantranilipole was 69 isolates. Further test of pesticide-decomposing potency found that 18 isolates able to decompose prophenophos with clear zone diameter of 1.03 – 3.15 cm, 13 isolates were able to decompose chlorantraniliprole with clear zone diameter of 0.2 – 3.1 cm, while 5 isolates were able to decompose both pesticides with clear zone of 1.25 – 4.0 cm. Keywords— endemic bacteria, pesticide decomposer, prophenophos, chlorantraniliprole, East Java