芒廷叶甲醇提取物对胶原蛋白生成的促进作用

THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION Pub Date : 2019-12-18 DOI:10.1063/1.5139830

A. Rahmawati, O. P. Astirin, A. Pangastuti

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引用次数: 1

摘要

皮肤弹性丧失是随着年龄增长而发生的必然过程。真皮成纤维细胞减少胶原蛋白的分泌量，使皮肤出现皱纹。蒙廷花含有高酚含量，有助于减少氧化应激，从而增加胶原蛋白的产生。本研究旨在评价鱿鱼叶甲醇提取物(MLME)对3T3成纤维细胞培养中胶原蛋白产量的影响。实验采用氯化铝(AlCl3)比色法和酶联免疫吸附试验。AlCl3比色法测定的总黄酮含量(TFC)为584.147±20.299 mg QE/100 g干重，ELISA法测定的胶原分泌量显示，MLME对培养的3T3成纤维细胞前胶原-1-前肽n端(P1NP)的增加呈剂量依赖性，具有统计学意义(p < 0.05)。皮肤弹性丧失是随着年龄增长而发生的必然过程。真皮成纤维细胞减少胶原蛋白的分泌量，使皮肤出现皱纹。蒙廷花含有高酚含量，有助于减少氧化应激，从而增加胶原蛋白的产生。本研究旨在评价鱿鱼叶甲醇提取物(MLME)对3T3成纤维细胞培养中胶原蛋白产量的影响。实验采用氯化铝(AlCl3)比色法和酶联免疫吸附试验。AlCl3比色法测定的总黄酮含量(TFC)为584.147±20.299 mg QE/100 g干重，ELISA法测定的胶原分泌量显示，MLME对培养的3T3成纤维细胞前胶原-1-前肽n端(P1NP)的增加呈剂量依赖性，具有统计学意义(p < 0.05)。

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The effect of Muntingia calabura L. leaves methanolic extract in increasing of collagen production

Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.Skin elasticity loss is the certain process that happen along with the age. Dermis fibroblast cells decrease the amount of collagen secretion which make a wrinkled appearance. Muntingia calabura L. has a high phenolic content that helps in decreasing oxidative stress thus intended increasing collagen production. This study aimed to evaluate the effect of M. calabura leaves methanolic extract (MLME) in increasing the amount of collagen production in 3T3 fibroblast cells culture. Tests were performed using the alumunium chloride (AlCl3) colorimetry assay and ELISA assay. The value of total flavonoid content (TFC) obtained through the AlCl3 colorimetry assay is 584.147 ± 20.299 mg QE/100 gram dry weight, while testing for the amount of collagen secreted with ELISA assay showed that MLME statistically had significant effect (p < 0.05) in increasing procollagen-1-propeptide N-terminal (P1NP) of 3T3 fibroblast cells culture based on dose- dependent manner.

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THE 2ND INTERNATIONAL CONFERENCE ON SCIENCE, MATHEMATICS, ENVIRONMENT, AND EDUCATION

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