Short Term Effect of Plant Hybridosomes on Growth of Phaeodactylum Tricornutum Culture

Microalgae are in focus of extensive study due to their abundance and important role in equilibration of the global ecosystem. Living organisms communicate through nano-sized membrane-enclosed particles which are continuously shed by cells but can also be fabri-cated artificially. In this work we examined the effect of hybridosomes composed from soyabean lecithin, aqueous solution containing substances from spruce needles and glyc-erol and hybridosomes composed from soyabean lecithin, ultraclean water and hemp oil on the number density of microalgae Phaeodactylum tricornutum in culture. We measured the number density of microalgae by flow cytometry and the number density and hydro-dynamic diameter of small particles in the samples by interferometric light microscopy. We observed considerable increase of the number density of microalgae with respect to control (untreated) samples after three days, which was connected to the amount of the material added. Addition of membrane-enclosed particles had a favourable effect on the microalgae growth. Microalgae proved a convenient system for in vitro studies of the ef-fects of substances. Keywords: Phaeodactylum tricornutum; Hybridosomes; Liposomes; Nanoalgosomes; Ex-tracellular vesicles; Small cellular particles; Microalgae