一种测定α的简便方法。-淀粉酶使用微孔板。

Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry Pub Date : 1998-12-01 DOI:10.1271/NOGEIKAGAKU1924.72.933

Toshiya Satoyama, T. Hara, M. Murata, Y. Fujio

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引用次数: 5

摘要

本研究提出了一种新的α-淀粉酶活性测定方法。将α-淀粉酶原液加入琼脂和糯米淀粉的混合物中，固定在微孔板中，α-淀粉酶作用于糯米淀粉，降低了655 nm处的凝胶浊度。α-淀粉酶活性的强度与微孔板阅读器检测到的浊度降低成对数比例。所得数据的标准差和变异系数(3.7￣8.9%)合理，为α-淀粉酶活性的测定提供了方便。

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A Simple Assay Method for .ALPHA.-Amylase Using Microplates.

A novel method for measuring α-amylase-activity was developed in this study. By adding an authentic α-amylase solution to a mixture of agar and gelatinized rice starch, which was fixed in a well of a microplate, the gel turbidity measured at 655 nm was decreased by the α-amylase action on the gelatinized starch. The intensity of the α-amylase-activity was in proportion logarithmically to the turbidity decrease detected by a microplate-reader. From reasonable values of standard deviation and coefficient of variation (3.7_??_8.9%) of the obtained data, this method makes it possible to measure α-amylase-activity conveniently.

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Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry

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