gbobonise Epa Ijebu中药对泌尿系病原菌的微生物质量及疗效评价

Enitan S. S, Uduchukwu O. E, Gotep J, Effiong E. J, Ileoma E. O, Mensah-Agyei G. O, Adetiloro E. O, Adekunbi O. A, Odigie J. O, Adetola A. O
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Data generated were analyzed using the Instant GraphPad software package and P value <0.05 was considered statistically significant. Results: The outcome of the study shows that half (50%) of the herbal remedy samples examined were sterile, while the other half (50%) were contaminated with a microbial count of <10 CFU/mL. The microbial isolates recovered from the herbal remedy include: Staphylococcus aureus (8.3%), Coagulase negative Staphylococcus (75%) and Microsporium spp (16.7%). Assessment of the antimicrobial potential of the herbal remedy show that the test herbal remedy had strong inhibitory activity against Staphylococcus aureus (17±0.6 mm), Escherichia coli (16.3±0.3 mm), Pseudomonas aeruginosa (16.3±0.3 mm), Klebsiella pneumoniae (15±0.6 mm) and Proteus mirabilis (14±0.8 mm), except Candida albican, at a concentration of 200 mg/mL. 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引用次数: 0

摘要

背景:Gbogbonise Epa Ijebu是尼日利亚西南部一种商业销售的本地混合物,正如制造商和供应商所声称的那样,具有治疗许多疾病的潜力。本研究考察了青豆的抗菌质量和抗菌效果。材料和方法:共从尼日利亚奥贡州Ilishan-Remo社区的当地供应商处购买了20份草药样品,并使用标准微生物学方法对其微生物质量进行了评估。同时,采用冲孔琼脂扩散法对所选尿路病原菌进行药效试验。生成的数据采用Instant GraphPad软件包进行分析,P值<0.05为差异有统计学意义。结果:研究结果显示,半数(50%)被检查的中药样品是无菌的,而另一半(50%)被微生物计数<10 CFU/mL污染。从中药中分离出的微生物包括:金黄色葡萄球菌(8.3%)、凝固酶阴性葡萄球菌(75%)和小孢子菌(16.7%)。抑菌潜力评价表明,在浓度为200 mg/mL时,除白色念珠菌外,对金黄色葡萄球菌(17±0.6 mm)、大肠杆菌(16.3±0.3 mm)、铜绿假单胞菌(16.3±0.3 mm)、肺炎克雷伯菌(15±0.6 mm)和神奇变形杆菌(14±0.8 mm)均有较强的抑菌活性。除奇异变形杆菌外,复方药液与标准药的抑制带直径较高:肺炎克雷伯菌(25±0.6 mm)、铜绿假单胞菌(24±0.6 mm)、金黄色葡萄球菌(23.7±0.3 mm)、大肠杆菌(23.3±0.3 mm)、白色念珠菌(15±0.6mm)。除白色念珠菌外,所有菌株的最小抑菌浓度(MIC)和最小杀菌/杀真菌浓度(MBC/MFC)分别为100mg/mL和200mg/mL。植物化学筛选结果显示,该植物含有生物碱、蒽醌类、心苷类、黄酮类、皂苷类和单宁类,不含类固醇和树脂。结论:本研究结果进一步强化了中药生产商和销售商所宣称的疗效。然而,考虑到消费者的总体安全,应更多地关注质量准备、包装和储存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Assessment of Microbiological Quality and Efficacy of Gbogbonise Epa Ijebu Herbal Remedy on Some Uropathogens
Background: Gbogbonise Epa Ijebu is a commercially sold native concoction in South-West, Nigeria, with the potential of curing many ailments as claimed by the manufacturer and vendors. This study investigated the antimicrobial quality and efficacy of Gbogbonise Epa Ijebu. Materials and Methods: A total of 20 samples of the herbal remedy were purchased from local vendors in Ilishan-Remo Community of Ogun State, Nigeria and were assessed for their microbiological quality using standard microbiological methods. Also, the efficacy of the herbal remedy was tested against some selected uropathogens using the Punch-hole agar diffusion method. Data generated were analyzed using the Instant GraphPad software package and P value <0.05 was considered statistically significant. Results: The outcome of the study shows that half (50%) of the herbal remedy samples examined were sterile, while the other half (50%) were contaminated with a microbial count of <10 CFU/mL. The microbial isolates recovered from the herbal remedy include: Staphylococcus aureus (8.3%), Coagulase negative Staphylococcus (75%) and Microsporium spp (16.7%). Assessment of the antimicrobial potential of the herbal remedy show that the test herbal remedy had strong inhibitory activity against Staphylococcus aureus (17±0.6 mm), Escherichia coli (16.3±0.3 mm), Pseudomonas aeruginosa (16.3±0.3 mm), Klebsiella pneumoniae (15±0.6 mm) and Proteus mirabilis (14±0.8 mm), except Candida albican, at a concentration of 200 mg/mL. The combined herbal solution and standard drug gave higher zone diameter of inhibition: Klebsiella pneumoniae (25±0.6 mm), Pseudomonas aeruginosa (24±0.6 mm), Staphylococcus aureus (23.7±0.3 mm), Escherichia coli (23.3±0.3 mm) and Candida albican (15±0.6mm), except Proteus mirabilis. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC) of the herbal remedy for all the test isolates (except Candida albican) was 100mg/mL and 200mg/mL, respectively. The result of the phytochemical screening showed the presence of alkaloids, anthraquinones, cardiac glycosides, flavonoids, saponins and tannins, while steroids and resins were absent. Conclusion: The outcome of this study further strengthens the claim of efficacy made by the manufacturer and vendors of the herbal remedy. However, more attention should be given to quality preparations, packaging and storage in view of the general safety of the consumers.
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