EFFICIENT IN VITRO PLANTLET REGENERATION FROM COTYLEDON EXPLANTS IN SUGAR BEET (BETA VULGARIS L.)

An efficient in vitro regeneration technique was established in sugar beet (Beta vulgaris L.) using cotyledon as explants from in vitro raised seedlings. Regarding plant growth regulators added in inoculation media showed that BA in combinations with NAA was most responsive for multiple shooting in which, 90% of shoot emergence from inoculated explants were observed with a medium containing 0.5mg/L BA+0.5mg/L NAA at 30 days of inoculation period. The highest number of shoots per explant was 7.5? 0.56 and the maximum shoot length of 6.5?0.25cmwere observed in this medium inoculation after 90 days. In vitro raised shoots rooted well when they were transferred to MS + 3.0mg/LIBA in which, 70% shoots were rooted in this medium transferring after 30 days. The maximum number of roots per shoot was 5.50?0.46 and the average root length of 5.8 ?0.62 cm were recorded inoculation after 45 days. About6 months were needed from culture initiation to plantlets establishment in natural environment and 55% plantlets were found to be acclimatized and resumed a new growth.