{"title":"年龄和AgNor-一项形态计量学研究","authors":"Manpreet Kaur, Smita Naik, Sachin Jindal","doi":"10.52152/SPR.2020.01.106","DOIUrl":null,"url":null,"abstract":"To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA ) values in buccal epithelial cells of healthy individuals in different age groups. Material and Methodology:- In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups.Group1-10-20year, Group2-20- 30year, Group3-30-40year, Group4-40-50year, Group5-50-60year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Results: Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. Conclusion: There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.","PeriodicalId":162349,"journal":{"name":"Science Progress and Research","volume":"70 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Age and AgNor- A Morphometric study\",\"authors\":\"Manpreet Kaur, Smita Naik, Sachin Jindal\",\"doi\":\"10.52152/SPR.2020.01.106\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA ) values in buccal epithelial cells of healthy individuals in different age groups. Material and Methodology:- In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups.Group1-10-20year, Group2-20- 30year, Group3-30-40year, Group4-40-50year, Group5-50-60year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Results: Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. Conclusion: There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.\",\"PeriodicalId\":162349,\"journal\":{\"name\":\"Science Progress and Research\",\"volume\":\"70 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2020-12-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Science Progress and Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.52152/SPR.2020.01.106\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Science Progress and Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.52152/SPR.2020.01.106","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
To investigate and correlate total AgNOR area/Total nucleus area (TAA/TNA ) values in buccal epithelial cells of healthy individuals in different age groups. Material and Methodology:- In present study 50 healthy individuals are included with age ranging 10 to 60 years. These are divided into 5 groups.Group1-10-20year, Group2-20- 30year, Group3-30-40year, Group4-40-50year, Group5-50-60year. Oral epithelial cells collected with the help of cyto brush from buccal mucosa. Smears were prepared on clean glass slides and fixed with 95% alcohol. Fixed slides Smears were stained with AgNOR stain. Results: Statistically significant correlations were found between mean TAA/TNA values and age p < 0.001 for linear and p < 0.0001 for polynominal regression, and between AgNOR number and age, p < 0.001 for linear. Conclusion: There is a significant correlation between age and AgNOR amount (ribosome biosynthesis rate) in buccal epithelial cells of healthy individuals. AgNORs in buccal epithelial cells may be used for detection of age.
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