{"title":"通过薄层色谱法进行Grayanotoxin I, II和III的定量","authors":"Tadamasa Terai","doi":"10.1271/NOGEIKAGAKU1924.58.1117","DOIUrl":null,"url":null,"abstract":"A thin-layer chromatographic method was developed for identifying grayanotoxin (G)-I, G-II and G-III, which are toxic diterpenoids of Leucothoe grayana MAXIM. The optimum onditions for quantitative analysis were as follows: developing solvent, EtOAc: MeOH=37:3; TLC plate, Merck Kieselgel 60 containing 40% H2O; detection agent, 20% phosphotungstic acid in ethanol; charring temperature and time, 90°C and 20 min. Mixtures of G-1, G-II and G-III were analyzed under these conditions, and reliable results were obtained. This method was applied for identifying grayanoid compounds in toxic honey.","PeriodicalId":170824,"journal":{"name":"Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry","volume":"16 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1984-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"薄層クロマトグラフィーによるGrayanotoxin I, IIおよびIIIの定量\",\"authors\":\"Tadamasa Terai\",\"doi\":\"10.1271/NOGEIKAGAKU1924.58.1117\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A thin-layer chromatographic method was developed for identifying grayanotoxin (G)-I, G-II and G-III, which are toxic diterpenoids of Leucothoe grayana MAXIM. The optimum onditions for quantitative analysis were as follows: developing solvent, EtOAc: MeOH=37:3; TLC plate, Merck Kieselgel 60 containing 40% H2O; detection agent, 20% phosphotungstic acid in ethanol; charring temperature and time, 90°C and 20 min. Mixtures of G-1, G-II and G-III were analyzed under these conditions, and reliable results were obtained. This method was applied for identifying grayanoid compounds in toxic honey.\",\"PeriodicalId\":170824,\"journal\":{\"name\":\"Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry\",\"volume\":\"16 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1984-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1271/NOGEIKAGAKU1924.58.1117\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nippon Nogeikagaku Kaishi-journal of The Japan Society for Bioscience Biotechnology and Agrochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1271/NOGEIKAGAKU1924.58.1117","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A thin-layer chromatographic method was developed for identifying grayanotoxin (G)-I, G-II and G-III, which are toxic diterpenoids of Leucothoe grayana MAXIM. The optimum onditions for quantitative analysis were as follows: developing solvent, EtOAc: MeOH=37:3; TLC plate, Merck Kieselgel 60 containing 40% H2O; detection agent, 20% phosphotungstic acid in ethanol; charring temperature and time, 90°C and 20 min. Mixtures of G-1, G-II and G-III were analyzed under these conditions, and reliable results were obtained. This method was applied for identifying grayanoid compounds in toxic honey.
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