Toluidine blue-alizarin red staining of the forelimb bones of 24 days old rabbit embryos (Oryctolagus Cuniculus)

Skeleton is a supporting system for vertebrate body. It provides attachments for muscles, houses the brain and the spinal cord, and serves for locomotion.1 Staining of animal skeleton is important in anatomical studies. Toluidine Blue-Alizarin Red staining is one of the most successful staining methods for foetal and young skeletons. Some authors have described the skeletal examination of animals by Alizarin Staining2 but there is scarcity with regard to staining of appendicular system in rabbits.