应用μLAS技术直接表征血浆循环DNA

2017 IEEE International Electron Devices Meeting (IEDM) Pub Date : 2017-12-02 DOI:10.1109/IEDM.2017.8268465

R. Malbec, B. Chami, H. T. M. Ngo, A. Didelot, F. Garlan, S. Garrigou, V. Taly, Lorène Aeschbach, Evgeniya Trofimenko, Vincent Dion, A. Boutonnet-Rodat, F. Ginot, A. Bancaud

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引用次数: 1

摘要

循环无细胞DNA (cfDNA)是建立靶向治疗或监测患者治疗的强有力的癌症生物标志物。然而，目前的cfDNA鉴定受到其低浓度的严重限制，需要广泛使用扩增技术。在这里，我们报告了μLAS技术使我们能够在几分钟内定量表征纯化cfDNA的大小分布，即使其浓度低至1 pg/μL。此外，我们表明DNA谱可以在血浆中直接测量，只需最小的调节过程，从而大大加快cfDNA分析链的速度。

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Direct characterization of circulating DNA in blood plasma using μLAS technology

Circulating cell-free DNA (cfDNA) is a powerful cancer biomarker for establishing targeted therapies or monitoring patients' treatment. However, current cfDNA characterization is severely limited by its low concentration, requiring the extensive use of amplification techniques. Here we report that the μLAS technology allows us to quantitatively characterize the size distribution of purified cfDNA in a few minutes, even when its concentration is as low as 1 pg/μL. Moreover, we show that DNA profiles can be directly measured in blood plasma with a minimal conditioning process to speed up considerably speed up the cfDNA analytical chain.

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2017 IEEE International Electron Devices Meeting (IEDM)

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