马铃薯块茎蛾卵期对球孢白僵菌的敏感性

Nisreen Houssain Alsaoud, Doummar Hashim Nammour, Ali Yaseen Ali
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引用次数: 1

摘要

球孢白僵菌(Beauveria bassiana, Bals.)的3个地方分离株的致病性。对马铃薯块茎蛾卵进行了Vuill评价。3株分离株编码如下:B(来自拉塔基亚的分离株)、C(来自ICARDA的分离株)和D(来自大马士革的分离株)。每种分离物分别采用4、5、6三个浓度,分生菌数/ml。对照组用消过毒的水喷洒鸡蛋。所有试验均在温度28±2℃,相对湿度40±5%的实验室条件下进行。药敏试验结果显示,当1 × 10 6分生孢子/ml时,对照组与B、C分离菌的平均孵化率差异显著,分别为18.3%和26.6%,而D分离菌的平均孵化率为38.3%,对照组为66.6%。结果表明,球孢白僵菌的卵对球孢白僵菌的本地分离株有不同程度的敏感性,但在自然条件下,有效分离株对球孢白僵菌卵的控制效果有待进一步研究。C分别。药敏试验结果显示,当1 × 10 6分生孢子/ml时,对照与B、C菌株的平均孵化率差异显著,分别为18.3%和26.6%,而D菌株和对照的平均孵化率分别为38.3%和66.6%。结果表明,包壳假单胞菌的卵对当地球孢白僵菌有不同程度的敏感。研究结果鼓励进一步研究有效分离物在贮藏和田间自然条件下控制虫卵的效果,并在比本研究条件更好的其他阶段(成虫和幼虫)对当地分离物进行试验。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Susceptibility of Egg Stage of Potato Tuber Moth Phthorimaea operculella to Native Isolates of Beauveria bassiana
The pathogenicity of three local isolates of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill was evaluated on eggs of potato tuber moth P. operculella (Zeller). The three isolates were coded as the following: B (isolate from Latakia), C (isolate from ICARDA) and D (isolate from Damascus). Three concentrations 10 4 , 10 5 , and 10 6 , respectively, conidia/ml were used for each isolate. Eggs in the control were sprayed by sterilized water. All tests were done under laboratory conditions of temperature 28 ± 2°C and relative humidity 40 ± 5%. Susceptibility tests showed significant differences in averages of hatching rate between the control and both isolates B and C when 1 × 10 6 conidia/ml was applied, with averages 18.3 and 26.6% for previous isolates respectively, in contrast to 38.3 for isolate D and 66.6% for control. Findings indicated that eggs of P. operculella seemed sensible to local isolates of B. bassiana in varying degree, but further studies are required about the efficiency of effective isolates for controlling eggs of this pest in natural conditions. C respectively. Susceptibility tests showed significant differences in averages of hatching rate between the control and both isolates B and C when 1 × 10 6 conidia/ml was applied, with averages 18.3 and 26.6% for previous isolates respectively, in contrast 38.3% for isolate D and 66.6% for control. Findings indicated that eggs of P. operculella seemed sensible to local isolates of B. bassiana in varying degree. Results encourage further studies about the efficiency of effective isolates for controlling eggs of this pest in natural conditions of store and field and testing the local isolates on the other stages (adults and larvae) under better condition than this research condition.
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