滤波器在荧光分析仪器模拟和优化中的影响:SLAP

IEEE Sensors, 2005. Pub Date : 2005-10-31 DOI:10.1109/ICSENS.2005.1597849

S.-H.M. Liu, L. Lee, D. Wilson

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引用次数: 0

摘要

改进了一个综合模拟程序，模拟LED聚合程序(SLAP)，用于荧光分析系统的设计和优化已经实现。SLAP中包含的激发和发射滤波器使用户能够更灵活地设计荧光分析系统，以便在基于模块化led的架构中实现。对程序的改进允许用户在优化发射信号的两个主要特性(总信号强度和光谱重叠或与激发信号的干扰)时考虑样品、光路和滤波器。使用EGFP作为目标荧光团和XF100-2 Omega Optics滤波器组的代表性结果表明，干扰改善了99.7%，而总发射信号损失仅为70%。SLAP在潜在系统配置的综合分析中精确指出了总信号和干扰(噪声)之间的最佳权衡，以满足目标荧光分析仪器的需求

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Impact of filters in the simulation and optimization of fluorescence analysis instrumentation: SLAP

Improvements to a comprehensive simulation program, simulated LED aggregation program (SLAP), for the design and optimization of fluorescence analysis systems have been implemented. The inclusion of excitation and emission filters in SLAP enables the user increased flexibility in designing a fluorescence analysis system to be implemented in a modular LED-based architecture. Improvements to the program allow the user to consider the sample, optical path, and filters in the optimization of the two primary characteristics of the emission signal (total signal strength and spectral overlap or interference with the excitation signal). Representative results using EGFP as the targeted fluorophore and an XF100-2 Omega Optics filter set demonstrate a 99.7% improvement in interference with only a 70% loss in total emission signal. SLAP pinpoints the optimal trade-off between total signal and interference (noise) in a comprehensive analysis of potential system configurations to meet targeted fluorescence analysis instrumentation needs

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IEEE Sensors, 2005.

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