Minfeng Lu , Jinhui Chen , Han Meng , Guangling Mo , Yunhong Liu , Fengping Chen , Zonghua Wang , Mo Wang
{"title":"水稻铁氧还蛋白 OsFd4 有助于耐受氧化胁迫,但会削弱对枯萎病菌的防御能力","authors":"Minfeng Lu , Jinhui Chen , Han Meng , Guangling Mo , Yunhong Liu , Fengping Chen , Zonghua Wang , Mo Wang","doi":"10.1016/j.cj.2023.04.003","DOIUrl":null,"url":null,"abstract":"<div><p>Ferredoxins (Fds) in plastids are the most upstream stromal electron receptors shuttling electrons to downstream metabolic systems and function in various physiological processes of dicots, but their roles in monocots’ response to stresses are still unclear. In this study, the functions of OsFd4, the major non-photosynthetic type Fd in rice, were characterized under oxidative stress and <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> (<em>Xoo</em>) infection. <em>OsFd4</em>-knockout mutants displayed no defects in key agronomic traits and blast resistance, but were more sensitive to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment than the wild type. Transient expression of <em>OsFd4</em> alleviated H<sub>2</sub>O<sub>2</sub>-induced rice cell death, suggesting that OsFd4 contributes to rice tolerance to exogenous oxidative stress. Deletion of OsFd4 enhanced rice immune responses against <em>Xoo</em>. OsFd4 formed a complex <em>in vivo</em> with itself and OsFd1, the major photosynthetic Fd in rice, and <em>OsFd1</em> transcripts were increased in leaf and root tissues of the <em>OsFd4</em>-knockout mutants. These results indicate that OsFd4 is involved in regulating rice defense against stresses and interplays with OsFd1.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 6","pages":"Pages 1937-1942"},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123000521/pdfft?md5=3831176fc4de0b4915d6124f89293137&pid=1-s2.0-S2214514123000521-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria\",\"authors\":\"Minfeng Lu , Jinhui Chen , Han Meng , Guangling Mo , Yunhong Liu , Fengping Chen , Zonghua Wang , Mo Wang\",\"doi\":\"10.1016/j.cj.2023.04.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ferredoxins (Fds) in plastids are the most upstream stromal electron receptors shuttling electrons to downstream metabolic systems and function in various physiological processes of dicots, but their roles in monocots’ response to stresses are still unclear. In this study, the functions of OsFd4, the major non-photosynthetic type Fd in rice, were characterized under oxidative stress and <em>Xanthomonas oryzae</em> pv. <em>oryzae</em> (<em>Xoo</em>) infection. <em>OsFd4</em>-knockout mutants displayed no defects in key agronomic traits and blast resistance, but were more sensitive to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment than the wild type. Transient expression of <em>OsFd4</em> alleviated H<sub>2</sub>O<sub>2</sub>-induced rice cell death, suggesting that OsFd4 contributes to rice tolerance to exogenous oxidative stress. Deletion of OsFd4 enhanced rice immune responses against <em>Xoo</em>. OsFd4 formed a complex <em>in vivo</em> with itself and OsFd1, the major photosynthetic Fd in rice, and <em>OsFd1</em> transcripts were increased in leaf and root tissues of the <em>OsFd4</em>-knockout mutants. These results indicate that OsFd4 is involved in regulating rice defense against stresses and interplays with OsFd1.</p></div>\",\"PeriodicalId\":10790,\"journal\":{\"name\":\"Crop Journal\",\"volume\":\"11 6\",\"pages\":\"Pages 1937-1942\"},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214514123000521/pdfft?md5=3831176fc4de0b4915d6124f89293137&pid=1-s2.0-S2214514123000521-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Journal\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214514123000521\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123000521","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria
Ferredoxins (Fds) in plastids are the most upstream stromal electron receptors shuttling electrons to downstream metabolic systems and function in various physiological processes of dicots, but their roles in monocots’ response to stresses are still unclear. In this study, the functions of OsFd4, the major non-photosynthetic type Fd in rice, were characterized under oxidative stress and Xanthomonas oryzae pv. oryzae (Xoo) infection. OsFd4-knockout mutants displayed no defects in key agronomic traits and blast resistance, but were more sensitive to hydrogen peroxide (H2O2) treatment than the wild type. Transient expression of OsFd4 alleviated H2O2-induced rice cell death, suggesting that OsFd4 contributes to rice tolerance to exogenous oxidative stress. Deletion of OsFd4 enhanced rice immune responses against Xoo. OsFd4 formed a complex in vivo with itself and OsFd1, the major photosynthetic Fd in rice, and OsFd1 transcripts were increased in leaf and root tissues of the OsFd4-knockout mutants. These results indicate that OsFd4 is involved in regulating rice defense against stresses and interplays with OsFd1.
Crop JournalAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍:
The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics.
The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.