Marc Karle, J. Miwa, G. Roth, R. Zengerle, F. von Stetten
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A Novel Microfluidic Platform for Continuous DNA Extraction and Purification using Laminar Flow Magnetophoresis
We present a novel microfluidic platform using laminar-flow magnetophoresis for combined continuous extraction and purification of DNA. All essential unit operations (DNA binding, sample washing and DNA elution) are integrated on one single chip. The key function is the motion of magnetic beads given by the interplay of laminar flow and time-varying magnetic field. The time for extraction was 1 minute. The device is a central part of a complete biochemical system for continuous monitoring of cell-growth in bioreactors. The novel platform allows continuous purification of DNA, but is also applicable to purification of RNA, proteins or cells, including their subsequent real-time analysis in general.
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