Comparision of 10% Nitric Acid, Edta and 10% Formic Acid for Tooth Decalcification

Decalcification of hard tissues has remained an important part in visualizing the histology of the section. For the process of decalcification various decalcifying agents have been used in the past but very limited studies can be found in the literature about the comparison of various decalcifying agents. Aim: Here we present a study comparing three different decalcifying agents (10% Nitric acid, EDTA, 10 % Formic acid). Methodology: 30 freshly extracted premolars were decalcified using three different decalcifying agents. The end point of decalcification was tested by chemical methods. The decalcified sections were routinely processed and staining was done using Hematoxylin and Eosin. Result: Sections decalcified with EDTA gave better results as compared to the other two groups. Conclusion: EDTA is a better option for decalcification of tooth but it has a slow rate of action. So, for the cases requiring urgent results nitric acid can be used.