{"title":"抗原-抗体反应的计算机辅助分析","authors":"B. Liu, J. Steensgaard","doi":"10.1145/503561.503601","DOIUrl":null,"url":null,"abstract":"Antibodies are large molecules which are formed in the body to render antigens (causes of diseases) harmless. Bivalent antibodies and multivalent antigens may form a rich variety of different antigen-antibody complexes. The outcome of a reaction between an antigen and its antibodies can be analyzed experimentally by use of a gradient centrifugation technique.We have used computer models to study the extent to which the outcome of an antigen-antibody reaction is predictable from simple assumptions.First, a mathematical model for simulation of this particular centrifugal technique was constructed, allowing calculation of where in the centrifuge rotor a given complex would be located following centrifugation under precisely defined circumstances. Second, a program to predict the outcome of reactions between antigens and antibodies was written, based on the assumption that the kinds and the amounts of antigen-antibody complexes are determined by the concentrations of antigens and antibodies, respectively, together with a parameter describing their mutual affinity. Third, these programs were combined so as to calculate the expected outcome of antigen-antibody reactions as they appear after this type of centrifugal analysis. Finally, calculated results were compared to experimentally obtained results, and a fairly good agreement was found.It is concluded that the main characteristical features of such reactions are predictable from the above mentioned assumptions by use of these computer models.","PeriodicalId":151957,"journal":{"name":"ACM-SE 14","volume":"81 10","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1976-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Computer aided analysis of antigen-antibody reactions\",\"authors\":\"B. Liu, J. Steensgaard\",\"doi\":\"10.1145/503561.503601\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Antibodies are large molecules which are formed in the body to render antigens (causes of diseases) harmless. Bivalent antibodies and multivalent antigens may form a rich variety of different antigen-antibody complexes. The outcome of a reaction between an antigen and its antibodies can be analyzed experimentally by use of a gradient centrifugation technique.We have used computer models to study the extent to which the outcome of an antigen-antibody reaction is predictable from simple assumptions.First, a mathematical model for simulation of this particular centrifugal technique was constructed, allowing calculation of where in the centrifuge rotor a given complex would be located following centrifugation under precisely defined circumstances. Second, a program to predict the outcome of reactions between antigens and antibodies was written, based on the assumption that the kinds and the amounts of antigen-antibody complexes are determined by the concentrations of antigens and antibodies, respectively, together with a parameter describing their mutual affinity. Third, these programs were combined so as to calculate the expected outcome of antigen-antibody reactions as they appear after this type of centrifugal analysis. Finally, calculated results were compared to experimentally obtained results, and a fairly good agreement was found.It is concluded that the main characteristical features of such reactions are predictable from the above mentioned assumptions by use of these computer models.\",\"PeriodicalId\":151957,\"journal\":{\"name\":\"ACM-SE 14\",\"volume\":\"81 10\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1976-04-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACM-SE 14\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1145/503561.503601\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACM-SE 14","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1145/503561.503601","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Computer aided analysis of antigen-antibody reactions
Antibodies are large molecules which are formed in the body to render antigens (causes of diseases) harmless. Bivalent antibodies and multivalent antigens may form a rich variety of different antigen-antibody complexes. The outcome of a reaction between an antigen and its antibodies can be analyzed experimentally by use of a gradient centrifugation technique.We have used computer models to study the extent to which the outcome of an antigen-antibody reaction is predictable from simple assumptions.First, a mathematical model for simulation of this particular centrifugal technique was constructed, allowing calculation of where in the centrifuge rotor a given complex would be located following centrifugation under precisely defined circumstances. Second, a program to predict the outcome of reactions between antigens and antibodies was written, based on the assumption that the kinds and the amounts of antigen-antibody complexes are determined by the concentrations of antigens and antibodies, respectively, together with a parameter describing their mutual affinity. Third, these programs were combined so as to calculate the expected outcome of antigen-antibody reactions as they appear after this type of centrifugal analysis. Finally, calculated results were compared to experimentally obtained results, and a fairly good agreement was found.It is concluded that the main characteristical features of such reactions are predictable from the above mentioned assumptions by use of these computer models.