双接种番茄植株烟草花叶病毒轻、重度株的鉴定

IF 2.2 3区农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY

Annals of Applied Biology Pub Date : 1977-05-01 DOI:10.1111/j.1744-7348.1977.tb01813.x

A. C. CASSELLS, C. C. HERRICK

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引用次数: 9

摘要

利用特异性抗血清，用野生菌株攻毒后，可在番茄植株中鉴定出轻度(MII-16)和0、1株烟草花叶病毒(TMV)。此外，MII-16和mii - 0菌株的单损伤分离表明，基于局部损伤大小的差异，这两种菌株可以同时在混合分离中检测。通过将单个病损分离株反接种到感病番茄品种中，确定了这种局部病损鉴别方法的准确度为90%。

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The identification of mild and severe strains of tobacco mosaic virus in doubly inoculated tomato plants

Using specific antisera, it was possible to identify mild (MII-16) and the 0 and 1 strains of tobacco mosaic virus (TMV) in tomato plants infected with the mild strain following challenge inoculation with the wild strains.

In addition, with single lesion isolates of the MII-16 and o strains it has been shown that both strains can be assayed simultaneously in mixed isolates on Nicotiana glutinosa based on differences in local lesion size. The accuracy of this differential local lesion assay was c. 90% as determined by back inoculation of single lesion isolates into susceptible tomato cultivars.

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来源期刊

Annals of Applied Biology 生物-农业综合

CiteScore

5.50

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Annals of Applied Biology is an international journal sponsored by the Association of Applied Biologists. The journal publishes original research papers on all aspects of applied research on crop production, crop protection and the cropping ecosystem. The journal is published both online and in six printed issues per year. Annals papers must contribute substantially to the advancement of knowledge and may, among others, encompass the scientific disciplines of: Agronomy Agrometeorology Agrienvironmental sciences Applied genomics Applied metabolomics Applied proteomics Biodiversity Biological control Climate change Crop ecology Entomology Genetic manipulation Molecular biology Mycology Nematology Pests Plant pathology Plant breeding & genetics Plant physiology Post harvest biology Soil science Statistics Virology Weed biology Annals also welcomes reviews of interest in these subject areas. Reviews should be critical surveys of the field and offer new insights. All papers are subject to peer review. Papers must usually contribute substantially to the advancement of knowledge in applied biology but short papers discussing techniques or substantiated results, and reviews of current knowledge of interest to applied biologists will be considered for publication. Papers or reviews must not be offered to any other journal for prior or simultaneous publication and normally average seven printed pages.