{"title":"三叶根瘤菌和豆科根瘤菌噬菌体1P受体的研究。","authors":"E Zajac, R Russa, Z Lorkiewicz","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The rate of phage 1P attachment to Rhizobium cell walls was increased in the presence of ethylenediaminetetraacetic acid (EDTA). On the other hand the rate of adsorption of phage 1P to the Triton -- insoluble cell walls was diminished. The subsequent treatment of cell walls with 2% Triton X-100 and 5mM EDTA caused a more substantial decline of the phage inactivating capacity. Lipopolysaccharides (LPS) isolated from the sensitive strains, contrary to those from phage-resistant mutants, inactivated effectively phage 1P. The content of sugars in LPS preparations was determined by using gas liquid chromatography.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"7 4","pages":"181-8"},"PeriodicalIF":0.0000,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Studies on phage 1P receptors in Rhizobium trifolii and Rhizobium leguminosarum.\",\"authors\":\"E Zajac, R Russa, Z Lorkiewicz\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The rate of phage 1P attachment to Rhizobium cell walls was increased in the presence of ethylenediaminetetraacetic acid (EDTA). On the other hand the rate of adsorption of phage 1P to the Triton -- insoluble cell walls was diminished. The subsequent treatment of cell walls with 2% Triton X-100 and 5mM EDTA caused a more substantial decline of the phage inactivating capacity. Lipopolysaccharides (LPS) isolated from the sensitive strains, contrary to those from phage-resistant mutants, inactivated effectively phage 1P. The content of sugars in LPS preparations was determined by using gas liquid chromatography.</p>\",\"PeriodicalId\":75389,\"journal\":{\"name\":\"Acta microbiologica Polonica. Series A: Microbiologia generalis\",\"volume\":\"7 4\",\"pages\":\"181-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1975-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta microbiologica Polonica. Series A: Microbiologia generalis\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Polonica. Series A: Microbiologia generalis","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Studies on phage 1P receptors in Rhizobium trifolii and Rhizobium leguminosarum.
The rate of phage 1P attachment to Rhizobium cell walls was increased in the presence of ethylenediaminetetraacetic acid (EDTA). On the other hand the rate of adsorption of phage 1P to the Triton -- insoluble cell walls was diminished. The subsequent treatment of cell walls with 2% Triton X-100 and 5mM EDTA caused a more substantial decline of the phage inactivating capacity. Lipopolysaccharides (LPS) isolated from the sensitive strains, contrary to those from phage-resistant mutants, inactivated effectively phage 1P. The content of sugars in LPS preparations was determined by using gas liquid chromatography.
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