Sensor analysis of liquid pollution

The new portable microfluidic device for field measurement of organophosphorous pesticides toxicity is presented. The device consists of two loop pumps. The first loop assures the mixing of solution. The 95% of solution circulates through the first loop. The second loop consists of capillary which creates the hydrodynamic resistance and the chamber with the miniature electrochemical detector. The detection is based on the inhibition of acetylcholinesterase. The enzyme acetylcholinesterase is immobilized on the detector made by thick film technology forming together a biosensor. The analyzed sample is detected in the flow of supporting buffer. The biosensor detects the integral sample toxicity. The signal from biosensor is evaluated by Bioanalyzer electronic unit. It is destinated for detection of traces of pesticides in washout from leaves, for direct measurement in rivers, ponds, waste waters and drinking water sources.