蓝桉植物化学分析、生物活性测定及官能团检测

H. Gyawali, A. Ghimire, Toran Dip Dhital
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摘要

本研究旨在探讨蓝桉(Eucalyptus globulus)的植物化学筛选和抗菌活性。用索氏仪提取乙醇,热水提取水。植物化学筛选结果表明,乙醇提取物中含有生物碱、碳水化合物、皂苷、酚类、单宁、黄酮类、萜类、蛋白质和氨基酸、类固醇、挥发油;水提物中含有生物碱、碳水化合物、糖苷、皂苷、酚类、单宁、黄酮类、蛋白质和氨基酸、树胶和黏液、类固醇。采用琼脂孔扩散法进行抑菌活性试验。除乙醇提取物对大肠杆菌、葡萄球菌和芽孢杆菌有活性外,球芽孢杆菌乙醇提取物和水提物对大肠杆菌、葡萄球菌和芽孢杆菌均有抗性。球茎的醇提液和水提液对曲霉均无抑制作用。这项研究得出结论,从特定植物中提取的植物化学物质具有治疗危及生命的疾病的巨大潜力。红外光谱研究表明,植物提取物的主要成分为胺类、羧酸类、醇类和硝基化合物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical Analysis and Biological Activity Test and Functional Group Detection of Eucalyptus Globulus
This study was designed to explore phytochemical screening and antimicrobial activity of the Eucalyptus globulus. Extraction of phytochemical was performed by Soxhlet apparatus using ethanol and hot water extraction using water. The phytochemical screening of ethanolic extract showed the presence of alkaloids, carbohydrates, saponins, phenols, tannins, flavonoids, terpenoids, proteins & amino acids, steroids, volatile oils, and aqueous extract showed the presence of alkaloids, carbohydrates, glycosides, saponins, phenols, tannins, flavonoids, proteins & amino acids, gums and mucilage, and steroids. Agar well diffusion method was used for the antimicrobial activity test. Both ethanolic and aqueous extracts of E. globulus are resistant to the E. coli, Staphylococcus sp., and Bacillus species except ethanolic extract shows activeness against Staphylococcus sp. & Bacillus species. Both ethanolic and aqueous extracts of E. globulus show inactivity against Aspergillus species. This study concludes the phytochemicals derived from given plants has great potential to treat life-threatening disease. The FTIR studies show that plant extracts consist of amine, carboxylic acid, alcohol & nitro compounds as the most abundant component.
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