{"title":"产β-半乳糖苷酶乳酸菌的分离及培养基优化","authors":"Myung-Kuk Kim","doi":"10.29279/jitr.2022.27.4.35","DOIUrl":null,"url":null,"abstract":"Seven strains showing activity in MRS(De Man, Rogosa, and Sharpe) solid medium containing X-gal were selected from kimchi, yogurt, and earthworm feces. The strain isolated from yogurt showed the greatest activity. \nEnzyme activity was higher in cell suspensions than in cells or culture filtrates. The isolate was inferred to be a lactic acid bacterium based on Gram staining, biochemical tests, and the fact that it was isolated from commercial yogurt. The optimal concentrations of carbon, nitrogen, and minerals for cell growth and enzyme production were investigated. The results of this study are applicable to the production of β-galactosidase using lactic acid bacteria.","PeriodicalId":383838,"journal":{"name":"Korea Industrial Technology Convergence Society","volume":"44 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isolation and Media Optimization of Lactic Acid Bacteria Producing β-galactosidase\",\"authors\":\"Myung-Kuk Kim\",\"doi\":\"10.29279/jitr.2022.27.4.35\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Seven strains showing activity in MRS(De Man, Rogosa, and Sharpe) solid medium containing X-gal were selected from kimchi, yogurt, and earthworm feces. The strain isolated from yogurt showed the greatest activity. \\nEnzyme activity was higher in cell suspensions than in cells or culture filtrates. The isolate was inferred to be a lactic acid bacterium based on Gram staining, biochemical tests, and the fact that it was isolated from commercial yogurt. The optimal concentrations of carbon, nitrogen, and minerals for cell growth and enzyme production were investigated. The results of this study are applicable to the production of β-galactosidase using lactic acid bacteria.\",\"PeriodicalId\":383838,\"journal\":{\"name\":\"Korea Industrial Technology Convergence Society\",\"volume\":\"44 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Korea Industrial Technology Convergence Society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.29279/jitr.2022.27.4.35\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korea Industrial Technology Convergence Society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.29279/jitr.2022.27.4.35","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation and Media Optimization of Lactic Acid Bacteria Producing β-galactosidase
Seven strains showing activity in MRS(De Man, Rogosa, and Sharpe) solid medium containing X-gal were selected from kimchi, yogurt, and earthworm feces. The strain isolated from yogurt showed the greatest activity.
Enzyme activity was higher in cell suspensions than in cells or culture filtrates. The isolate was inferred to be a lactic acid bacterium based on Gram staining, biochemical tests, and the fact that it was isolated from commercial yogurt. The optimal concentrations of carbon, nitrogen, and minerals for cell growth and enzyme production were investigated. The results of this study are applicable to the production of β-galactosidase using lactic acid bacteria.
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