M. Rizk, Maha Abou El- alamin, S. Mowaka, Mariam Mohamed
{"title":"反相高效液相色谱法同时测定盐酸奈比洛尔、缬沙坦和氢氯噻嗪在抗高血压药物和血浆中的含量","authors":"M. Rizk, Maha Abou El- alamin, S. Mowaka, Mariam Mohamed","doi":"10.21608/bfsa.2022.167660.1537","DOIUrl":null,"url":null,"abstract":"Reversed phase high performance liquid chromatography combined with photodiode array (HPLC-PDA) detector method was developed for simultaneous determination of two binary antihypertensive combinations; either Nebivolol (NEB) with Hydrochlorothiazide (HCZ) or with Valsartan (VAL) in pharmaceutical formulation and spiked human plasma. The method was successfully applied to separate the three drugs on one chromatogram under the same chromatographic conditions that used for two binary combinations. The separation was established on Xterra C 18 column using gradient mobile phase which composed of acidified water of pH = 3.0 (adjusted with 0.1% orthophosphoric acid) and acetonitrile with flow rate of 1.0 mL/min. The drugs were determined at 280 nm. Concentration range of each analyte was shown linearity at 5.0–70.0 μg/mL for NEB, 8.0–60.0 μg/mL for HCZ and 5.0-80.0 μg/mL for VAL. The results of method validation were displayed according to ICH guidelines. The green assessment of the proposed has been carried out using two different tools.","PeriodicalId":345040,"journal":{"name":"Bulletin of Pharmaceutical Sciences. Assiut","volume":"12 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Development of RP-HPLC Method for Simultaneous Determination of Nebivolol Hydrochloride, Valsartan and Hydrochlorothiazide in co-administrated Antihypertensive Agents and Human Plasma\",\"authors\":\"M. Rizk, Maha Abou El- alamin, S. Mowaka, Mariam Mohamed\",\"doi\":\"10.21608/bfsa.2022.167660.1537\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Reversed phase high performance liquid chromatography combined with photodiode array (HPLC-PDA) detector method was developed for simultaneous determination of two binary antihypertensive combinations; either Nebivolol (NEB) with Hydrochlorothiazide (HCZ) or with Valsartan (VAL) in pharmaceutical formulation and spiked human plasma. The method was successfully applied to separate the three drugs on one chromatogram under the same chromatographic conditions that used for two binary combinations. The separation was established on Xterra C 18 column using gradient mobile phase which composed of acidified water of pH = 3.0 (adjusted with 0.1% orthophosphoric acid) and acetonitrile with flow rate of 1.0 mL/min. The drugs were determined at 280 nm. Concentration range of each analyte was shown linearity at 5.0–70.0 μg/mL for NEB, 8.0–60.0 μg/mL for HCZ and 5.0-80.0 μg/mL for VAL. The results of method validation were displayed according to ICH guidelines. The green assessment of the proposed has been carried out using two different tools.\",\"PeriodicalId\":345040,\"journal\":{\"name\":\"Bulletin of Pharmaceutical Sciences. Assiut\",\"volume\":\"12 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-11-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Pharmaceutical Sciences. Assiut\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21608/bfsa.2022.167660.1537\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Pharmaceutical Sciences. Assiut","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/bfsa.2022.167660.1537","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Development of RP-HPLC Method for Simultaneous Determination of Nebivolol Hydrochloride, Valsartan and Hydrochlorothiazide in co-administrated Antihypertensive Agents and Human Plasma
Reversed phase high performance liquid chromatography combined with photodiode array (HPLC-PDA) detector method was developed for simultaneous determination of two binary antihypertensive combinations; either Nebivolol (NEB) with Hydrochlorothiazide (HCZ) or with Valsartan (VAL) in pharmaceutical formulation and spiked human plasma. The method was successfully applied to separate the three drugs on one chromatogram under the same chromatographic conditions that used for two binary combinations. The separation was established on Xterra C 18 column using gradient mobile phase which composed of acidified water of pH = 3.0 (adjusted with 0.1% orthophosphoric acid) and acetonitrile with flow rate of 1.0 mL/min. The drugs were determined at 280 nm. Concentration range of each analyte was shown linearity at 5.0–70.0 μg/mL for NEB, 8.0–60.0 μg/mL for HCZ and 5.0-80.0 μg/mL for VAL. The results of method validation were displayed according to ICH guidelines. The green assessment of the proposed has been carried out using two different tools.