Anti-Bacterial Activity of Shadanga Ghrita & Its Ghana Vati on Diaarrhoea Causing Enteropathogens

Background: In Ayurvedic classics Diarrhoea is described with the name of Atisaar. In modern science it is closely correlated with watery Diarrhoea which is defined as having loose or watery stools at least three times per day or more frequently than normal for an individual. Diarrhoea caused by bacterial pathogens is a global health problem, especially in developing countries and enteric bacterial pathogens are the main cause of infectious Diarrhoea. Ayurvedic literature have lot of unexplored or least tested medicine, Shadanga Ghrita is one of those Ayurvedic formulation used in the management of Tridoshaja Atisaar. But it has some disadvantages such as bitterness in taste, unpalatability, feasibility and inconvenience in transportation. Considering these, here an attempt was made to transform it into a new dosage form i.e., Ghana Vati. Aim & Objectives: Aim & objectives of present study was to evaluate the anti-bacterial activity of Shadanga Ghrita and its new dosage form on Diarrhoea causing enteropathogens. Material and Methods: Samples of Shadanga Ghrita & its Ghana Vati were prepared as per Sharangdhar Samhita Madhyama Khanda. All the samples of finished products were analyzed on organoleptic and physico-chemical parameters. Anti-bacterial study was done by using Well Diffusion Method and Muller Hinton Agar Media was used to evaluate anti-bacterial activity against S. aureus, E. coli, S. enterica, S. boydii, Y. enterolitica, A. species, and C. perfringens. Result: Both samples showed significant anti-bacterial activity against Diarrhoea causing enteropathogens. Conclusion: Shadanga Ghrita is more effective Antibacterial agent, as it was formulated by Sneha Paka Kalpana method; Gou- Ghrita has potentiated the anti- bacterial effect of it.