K. Dowling, S. Hyde, N. Barry, J. Dainty, P. French, A. J. Hughes, M. Lever, A. Dymoke-Bradshaw, J. Hares, P. Kellett
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High-resolution, whole-field, fluorescence lifetime imaging of fluorophore distribution and environment
Fluorescence lifetime imaging (FLIM) can provide noninvasive functional/diagnostic imaging by exploiting the sensitivity of fluorescence lifetime to the environmental changes in, for example, ion concentration (e.g., Ca2+), and pH [e.g., Refs. 1 and 2].