自体外周血单个核细胞子宫输注后复发性着床失败患者子宫内膜中miR-199a-5p、miR-125b-5p及其靶基因的表达

Azita Azarpoor, A. Ardeshirylajimi, Samira Mohammadi-Yeganeh, Elham Pourmatrood, Z. Dehghan, Fattaneh Farifteh Nobijari, M. Salehi
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引用次数: 0

摘要

尽管生育技术取得了许多进步,但仍有一些人经历了植入失败。治疗方法之一是研究复发性植入失败(RIF)患者植入过程的免疫学方面。外周血单个核细胞(PBMC)疗法和富血小板血浆是目前可用的细胞疗法。本研究的目的是确定受miR-199a-5p和miR-125b-5p调控的FGFR-2和LIF基因的表达。这些基因在pbmc治疗的RIF患者的植入中起着基础作用。将20例临床诊断为RIF的患者随机分为RIF患者联合pbmc宫内输注组(n = 10)和RIF组(n = 10)。正常健康女性(n = 10)作为对照组。为了检验治疗组注射PBMCs的疗效,我们在三组中评估miR-199a-5p和miR-125-5p以及FGFR-2和LIF作为靶基因的表达,并比较结果。我们发现RIF组miR-199a-5p和miR-125-5p表达较高,其靶基因表达降低。然而,与RIF组相比,子宫内输注pbmc的RIF患者中FGFR-2和LIF基因的表达均升高,miR-199a-5p和miR-125b-5p的表达均显著降低。pbmc治疗可以有效地改变与子宫内膜容受性相关的microrna和基因的表达,并通过改变它们的表达及其在胚胎发育中的作用来改善这一过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expressions of miR-199a-5p and miR-125b-5p and their target genes in the endometrium of recurrent implantation failure patients following in uterus infusion of autologous peripheral blood mononuclear cells
Despite numerous advances in fertility techniques, some individuals experience implantation failure. One of the therapeutic approaches is the study of immunological aspects of the implantation process in recurrent implantation failure (RIF) patients. Peripheral blood mononuclear cell (PBMC) therapy and platelet-rich plasma are currently available cell therapies. The aim of this study was to determine the expressions of the FGFR-2 and LIF genes that are regulated by miR-199a-5p and miR-125b-5p. These genes play a fundamental role in implantation in RIF patients treated with PBMCs. 20 patients clinically diagnosed with RIF were randomly assigned to a RIF patient with PBMCs intrauterine infusion group (n = 10) and RIF group (n = 10). Normal, healthy females (n = 10) comprised the control group. In order to examine the efficacy of the PBMCs injection in the treatment group, expressions of miR-199a-5p and miR-125-5p and FGFR-2 and LIF as their target genes, were evaluated in all three groups and were compared the results. We discovered that the RIF group had higher expressions of miR-199a-5p and miR-125-5p along with decreased expressions of their target genes. However, both FGFR-2 and LIF gene had elevated expressions in the RIF patients with PBMCs intrauterine infusion group compared to the RIF group, with significant decrease in miR-199a-5p and miR-125b-5p reciprocally. The treatment with PBMCs can be effective in changing the expression of microRNAs and genes associated with endometrial receptivity and by changes in the expression of them and their role during embryo development improve this process.
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