建议摇晃不含添加剂的红帽采血管,以加速血液凝固过程

Gilang Nugraha, Rohayati Rohayati
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引用次数: 0

摘要

许多采血管生产厂家不建议摇晃红色的采血管。摇晃红色试管,以避免血液与试管玻璃表面相互作用的强度变化,这将触发凝血级联中的接触路径。一般来说,血液在室温下凝固需要30-60分钟。本研究的目的是确定摇红帽采血管产生血清容量的有效性。本研究的方法是实验性的,取5 mL的血液,装入3根管,每根管的体积为1mL。第一管和第二管摇8次。第一管孵育10分钟,第二管孵育25分钟。同时,第三根试管(作为对照)不摇晃,但孵育40分钟。3000g离心10分钟。用微量移液管测定血清体积,收集至埃彭多夫管。结果显示,摇管后血清形成数随时间变化有差异(P = 0.002),而采用Bonferroni检验的事后检验结果显示,第二管血清体积与对照组无差异(P = 0.751)。由此可见,红帽采血管摇8次,孵育25分钟,可加速凝血过程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SHAKING RED CAP-BLOOD COLLECTION TUBE WITHOUT ADDITIVE SUBSTANCES IS RECOMMENDED TO ACCELERATE THE BLOOD CLOTTING PROCESS
Many blood collection tube manufacturers do not recommend shaking the red tube. Shaking the red tube to avoid the intensity of the changed of blood that interacts with the glass surface of the tube will trigger the contact path in the coagulation cascade. Generally, the blood takes 30-60 minutes to clots at room temperature without shaking. The aim of this study was to determine the effectiveness of shaking the red-cap blood collection tube in producing serum volume. The method of this study was experimental As much as 5 mL of blood was taken and put into 3 tubes with a volume of 1mL each tube. The first and the second tube were shaken 8 times. The first tube was incubated for 10 minutes while the second tube was incubated for 25 minutes. Meanwhile, the third tube (as a control) was not shaken but was incubated for 40 minutes. The tube were centrifuged at 3000 g for 10 minutes. The serum volume was measured using micropipette and collected into Eppendorf tube. The results showed that there were a difference in the number of serums formed after tube shaking by time variation (P = 0.002), the results of the Post Hoc Test using Bonferroni test while showed that the second tube did not have a difference in serum volume with control (P = 0.751). It can be concluded that the red-cap blood collection tube, which was shaken 8 times and incubated for 25 minutes long could accelerate the coagulation process.
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