用于光动力尿液诊断的微流控细胞分选器

The 7th IEEE International Conference on Nano/Molecular Medicine and Engineering Pub Date : 2013-11-01 DOI:10.1109/NANOMED.2013.6766309

D. Takagi, Y. Hirai, T. Tsuchiya, O. Tabata, S. Anai, Y. Chihara, K. Fujimoto, Y. Hirao

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引用次数: 1

摘要

我们报道了一种基于尿液光动力学分析的用于泌尿生殖系统癌早期诊断的新型微流控细胞分选系统。在已开发的系统中，利用5-氨基乙酰丙酸(5-ALA)诱导荧光检测泌尿生殖系统癌细胞，然后进行基因改变分析。这种细胞分选器的独特之处在于，微通道的微观结构是用一步紫外光刻技术制造的，从而创造了一种一次性的、低成本的、具有低自身荧光的设备。在本文中，我们研究了微流控细胞分选系统诊断泌尿生殖系统癌的性能，并评估了使用5-ALA治疗和使用荧光珠时癌细胞分离的准确性。

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Microfluidic cell sorter for photodynamic urine diagnosis

We report a new microfluidic cell sorting system for early diagnosis of genitourinary cancer based on photodynamic analysis of urine. In the developed system, genitourinary cancer cells are detected using 5-aminolevulinic acid (5-ALA)-induced fluorescence and then sorted for gene alteration analysis. The unique characteristic of this cell sorter is that the microchannel's microstructure is fabricated using one-step UV photolithography, to create a disposable, low-cost device that has low autofluorescence. In this paper, we examine the performance of the microfluidic cell sorting system for diagnosing genitourinary cancer, and evaluate the accuracy of cancer cell separation when using the 5-ALA treatment, and also when using fluorescent beads.

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The 7th IEEE International Conference on Nano/Molecular Medicine and Engineering

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