巨噬细胞调节淋巴细胞对植物血凝素、豆豆蛋白A和半异体细胞的反应。

E Hem
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引用次数: 0

摘要

为了研究巨噬细胞对植物血凝素(PHA)、魔豆蛋白A (Con A)和半同种异体细胞(半同种异体细胞)激活大鼠淋巴细胞的影响,我们在淋巴结淋巴细胞培养中加入浓度增加的腹膜细胞(PC)。淋巴结细胞制剂含有2-4%酯酶阳性细胞(巨噬细胞/单核细胞)。任何额外的PC似乎只抑制MLR,而PHA和Con A刺激培养的最佳反应分别发生在添加1-2%和3-7% PC时。在孵育的最后24小时,添加10%的PC显著抑制PHA和Con A刺激培养的反应。抑制细胞属于PC的玻璃棉贴壁群体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Modulation by macrophages of lymphocyte responses to phytohaemagglutinin, concanavalin A and semiallogeneic cells.

To investigate the effect of macrophages on rat lymphocyte activation by phytohaemagglutinin (PHA), concanavalin A (Con A) and semiallogeneic cells (uniderectional MLR), increasing concentrations of peritoneal cells (PC) were added to cultures of lymph node lymphocytes. Lymph node cell preparations contained 2-4% esterase positive cells (macrophages/monocytes). Any additional PC seemed only to inhibit MLR, whereas optimal responses of PHA and Con A stimulated cultures occurred when 1-2% abd 3-7% PC respectively were added. Ten percent PC markedly inhibited the response of both PHA and Con A stimulated cultures when added for the last 24 h of incubation. Inhibitory cells were shown to belong to the glass-wool adherent population of PC.

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